依达拉奉对脓毒血症大鼠肝及心肌线粒体SDH的影响  

The effect on succinate dehydrogenase of mitochondria in myocardium and liver in sepsis rats treated with edaravone

作　　者：缪心军[1] 陈红辉[1] 陈玉熹[1] 陈之力[1] 尤荣开[1] 

机构地区：[1]温州医学院定理临床学院、温州市第二人民医院急救科,浙江省温州325000

出　　处：《中国医师杂志》2010年第10期1301-1303,共3页Journal of Chinese Physician

基　　金：温州市科技局基金项目（Y20090163）,温州市卫生局基金项目（2007004）

摘　　要：目的 观察依达拉奉（ED）对脓毒血症大鼠肝及心肌线粒体琥珀酸脱氢酶（SDH）的影响.方法 SD大鼠30只随机分为假手术组（A组）、手术对照组（B组）、ED治疗组（C组）,B组和C组均予盲肠结扎穿刺法制作大鼠脓毒血症模型,三组术前15 min及术后3 h各皮下注射（sci）盐酸左氧氟沙星20 mg/kg,C组于术前15 min及术后3 h各sci ED 5 mg/kg,三组术后18 h取肝及心肌,测线粒体SDH活力,并对肝及心肌线粒体进行电镜检查.结果 B组肝及心肌线粒体SDH活力[（0.21±0.07）U/mgprot,（0.23±0.08）U/mgprot]显著低于A组[（0.33±0.10）U/mgprot,（0.38±0.12）U/mgprot];C组肝及心肌线粒体SDH活力[（0.31±0.08）U/mgprot,（0.36±0.11）U/mgprot]显著高于B组,B组电镜下可见肝及心肌线粒体轻度水肿,内质网扩张,C组心肌线粒体嵴多、致密.结论 脓毒血症大鼠肝及心肌线粒体结构破坏,SDH活力下降;ED能有效地升高其线粒体SDH活力,保护线粒体结构和功能.Objective To observe the effect on succinate dehydrogenase （SDH） of mitochondria in myocardium and liver in sepsis rats treated with edaravone. Methods 30 Sprague-Dawley rats were divided into 3 groups： sham operated group （ group A ）, controlled operated group （ group B ）, treated group with edaravone （group C）. The model of sepsis rats was made by the way of caecum ligated and punctured and 20mg/kg lactate levofloxacin was subcutaneously injected （sci） 15min before and 3h after operation in three group. 5mg/kg edaravone were sci 15min before and 3h after operation in group C. Liver and myocardium were taken from all of them 18h after operation. The activities of SDH in myocardial and hepatic mitochondria were detected, pathological change of mitochondria in liver and myocardium were observed. Results The activities of SDH in myocardial and hepatic mitochondria in group B [ （0. 21 ± 0. 07 ） U/mgprot, （0. 23± 0. 08 ） U/mgprot ] were significantly decreased compared with group A [ （ 0. 33 ± 0. 10 ） U/mgprot, （ 0. 38±0. 12）U/mgprot]. The activities of those in group C[ （0.31 ±0. 08） U/mgprot, （0. 36 ±0. 11）U/mgprot] were significantly increased than group B. Myocardial and hepatic mitochondria swelling and endocytoplasmic reticulum expanding were found in group B by electron microscope, while it showed normal in group C. Conclusion Hepatic and myocardial mitochondrial structure were destroyed and activities of SDH were decreased in sepsis rats. They could be effectively protected by edaravone.

关 键 词：安替比林/类似物和衍生物/药理学 脓毒症/药物疗法 线粒体 肝/药物作用 线粒体 心脏/药物作用 

分 类 号：R45[医药卫生—治疗学]