基于QCM-D的材料表面白蛋白吸附层随时间构象变化研究  

Conformation Changes with Time of Protein Adsorption Layer on Materials Surface Using Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)

作　　者：张奕[1] 焦延鹏[2] 周长忍[2] 薛巍[1] 

机构地区：[1]暨南大学生命与健康工程研究院药物载体实验室 [2]暨南大学材料科学与工程系,广州510632

出　　处：《中国生物医学工程学报》2010年第5期759-764,共6页Chinese Journal of Biomedical Engineering

基　　金：国家高新技术发展计划(863计划)项目(2007AA091603);广东自然科学基金(8151063201000075)

摘　　要：探讨生物材料表面白蛋白的吸附行为和构象变化。采用石英晶体微天平(QCM-D)、原子力显微镜和接触角等技术研究了聚乳酸、壳聚糖和空晶片等3种材料表面的亲疏水性及其表面的白蛋白吸附层粘弹性随时间变化规律。结果发现:这3种材料表面与水长时间接触后,疏水性顺序是:聚乳酸>空晶片>壳聚糖。长时间的白蛋白吸附实验显示,当吸附层质量达到平衡后,表征白蛋白粘弹性的|ΔD/ΔF|值随时间越来越大,并且在不同表面上的变化速率也不同。结果表明:白蛋白分子在材料表面发生与时间相关的构象铺展而造成蛋白层粘弹性随时间增大。并且疏水性越强的材料表面白蛋白吸附层的粘弹性变化越快。The adsorption behavior and conformational changes of protein plays a major role in determining the biocompatibility of materials.In this paper,the viscoelastic changes of bovine serum albumin（BSA）with time on poly lactic acid（PLA）,chitosan and Au were investigated using quartz crystal microbalance（QCM-D）,atomic force microscopy（AFM） and contact angle measurement.It was found that hydrophobic properties of the three materials was PLA Au Chitosan.During the BSA adsorption experiment for long time,｜ ΔD/ΔF ｜ value which was related to the viscoelastic property increased with the time after reaching adsorption equilibrium.Results revealed that protein molecules spread out with the adsorption time on the different surfaces which lead to viscoelastic changes of the protein layer.The change rate of the protein viscoelasticity showed a positive correlation with hydrophobicity of the materials surfaces.

关 键 词：生物材料 蛋白吸附 石英晶体微天平 吸附层粘弹性 亲疏水性 

分 类 号：O647.3[理学—物理化学]