促红细胞生成素对大鼠急性缺血视网膜节细胞的保护作用  

Protection of recombinant human erythropoietin on the retinal ganglion cells in retina ischemia reperfusion injury in rats

作　　者：丁国鹏[1] 沈学峰[2] 雷姝 毛治平[1] 任勇刚[1] 王涛[2] 张欲凯[2] 

机构地区：[1]中国陕西省宝鸡市人民医院眼科宝鸡市眼科中心,721000 [2]第四军医大学全军神经科学研究所,中国陕西省西安市710032 [3]中国陕西省宝鸡市妇幼医院药剂科,721000

出　　处：《国际眼科杂志》2010年第11期2071-2073,共3页International Eye Science

摘　　要：目的:探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对大鼠视网膜缺血再灌注损伤(retina ischemia reperfusion injury,RIR)中视网膜神经节细胞(retinal ganglion cell,RGC)的保护作用。方法:成年雌性SD大鼠20只,采用夹闭视网膜动脉30min造成大鼠双眼缺血再灌注模型。所有大鼠均于建模前1h给予左眼rhEPO10U(6μL),右眼给予同等剂量眼用平衡盐液。按照建模后眼球取材时间不同(1,4,7,14d)分为4组,每组5只,均于取材前4d利用荧光金(fluorogold,FG)逆行标记大鼠RGC,视网膜铺片RGC计数,比较双眼存活RGC数量。结果:rhEPO治疗眼RGC存活数多于平衡盐液对照眼。结论:rhEPO对大鼠视网膜急性缺血后RGC具有保护作用。AIM： To evaluate the effect of recombinant human erythropoietin （rhEPO） to the retinal ganglion cell （RGC） in retina ischemia reperfusion injury （RIR） in rats. METHODS： The RIR model was modeled by retinal artery＇s 30 minutes occlusion in 20 female SD rats. 6μL/10U rhEPO was injected in left eyes and physiological saline was injected in right eyes as vehicle controls at 1 hour before modeling. 20 rats were divided into four groups（1 day, 4,7,14 days）by the time of drawing the optic bulb after modeling. The survival RGCs was observed. The fluorogold （FG） tracing technique was used at 4 days before drawing the optic bulb in both eyes. RESULTS： The quantity of the RGCs in eyes treated with the rhEPO were significantly higher than those in control group. CONCLUSION： The rhEPO has protective effect on the RGCs in RIR in rats.

关 键 词：重组人促红细胞生成素 视网膜缺血再灌注损伤 视网膜神经节细胞 保护 

分 类 号：R96[医药卫生—药理学]