Identification of Fallopia Species Based on the Chloroplast matK Gene Sequences  

作　　者：SHENG Shujing YAN Ping ZHENG Chuanjin ZHAO Shujin 

机构地区：[1]School of Bioscience and Bioengineering, South ChinaUniversity of Technology, Guangzhou 510640, Guangdong, China [2]Department of Pharmacy, Guangzhou General Hospital ofGuangzhou Military Command, Guangzhou 510010, Guangdong, China

出　　处：《Wuhan University Journal of Natural Sciences》2010年第6期521-526,共6页武汉大学学报（自然科学英文版）

基　　金：Supported by the Natural Science Foundation of Guangdong Province(06019716)

摘　　要：In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.

关 键 词：FALLOPIA matK gene molecular identification 

分 类 号：S852.661[农业科学—基础兽医学] Q78[农业科学—兽医学]