产AmpC酶弗劳地枸橼酸杆菌的耐药基因研究  

Study on resistance gene of AmpC β-lactamase in Citrobacter freundii

作　　者：曾红莲 赵东明 徐韫健[2] 张晓坤[3] 

机构地区：[1]广州市白云区第一人民医院检验科,广东广州510000 [2]广州医学院第一附属医院检验科,广东广州510000 [3]广州医学院荔湾医院检验科,广东广州510000

出　　处：《中国热带医学》2011年第1期7-9,共3页China Tropical Medicine

基　　金：广东省科技厅项目(2008B060600046);广州医学院第一附属医院科研基金立项(编号:y200909gyfy)

摘　　要：目的对广州医学院第一附属医院临床分离的10株弗劳地枸橼酸杆菌进行AmpC酶的耐药基因研究。方法用超声破碎法提取10株菌的β-内酰胺酶粗提物,进行三维试验;提取10株细菌的总DNA,PCR扩增CMY-G1、CMY-G2、FOX、ACT、DHA、MOX、CIT耐药基因并进行测序;对菌株进行MIC药物敏感试验。结果三维试验阳性的有4株,β-内酰胺酶基因的检出率:CMY-G1(1/10)、CMY-G2(2/10)、FOX(1/10)、CIT(2/10),ACT、DHA和MOX未检出;CMY基因经全序列测定得到1 146bp的基因片段,与广州地区报道的CMY基因有99%同源;AmpC酶阳性的菌株菌呈多重耐药现象。结论加强菌弗劳地枸橼酸杆菌产AmpC酶的监测,以选择合适的抗生素应对产酶株。Aim To investigate the drug resistance gene of AmpC β-Lactamase from ten Citrobacter freundii. Methods -lactamases of ten strains was extracted by ultrasonic crushing,and AmpC -lactamases was tested with Cefoxitin in three dimensional test, the genotype of AmpC were amplified by multiple PCR and PCR products were sequenced subsequently. Drug sensitivity of ten strains were determined. Results There were 4 strains positive in three-dimensional test.The genotype of β-1aetamases were not detected in CMY-G1 （1/10）,CMY-G2 （2/10）,FOX （I/10）,CIT（2/10）,ACT, DHA and MOX genes in ten strains. The 1 146bp DNA fragment of CMY-type AmpC β-Laetamase gene sequence shared 99 % amino acid identity with blaCMY that already appeared in Guangzhou. The resistant strains overexpressing AmpC β-lactamases were multi-drug resistant. Conclusion The surveillance of overexpressing AmpC β-1actamases in Citrobacter freundii should be emphasized so as to choose effective antibiotic for dealing with AmpC β-1actamases producing strains.

关 键 词：AMPC酶 弗劳地枸橼酸杆菌 CMY 耐药性 

分 类 号：R37[医药卫生—病原生物学]