从人血液中克隆过氧化氢酶基因  

Cloning of Catalase Gene from Human Blood

作　　者：陈金峰[1] 胡斌杰[1] 程素满 

机构地区：[1]开封大学化学工程学院,河南省开封市475004 [2]开封市第五人民医院,河南省开封市475003

出　　处：《医学分子生物学杂志》2010年第5期410-413,共4页Journal of Medical Molecular Biology

基　　金：资助项目：河南省教育厅自然科学研究计划项目（No.2009C-180001）

摘　　要：目的从人血液中克隆过氧化氢酶基因。方法以新鲜的人血液为材料，提取全血RNA，运用RT—PCR方法扩增过氧化氢酶基因，构建pMDl8T／CAT克隆载体，并进行了不同来源过氧化氢酶的氨基酸序列同源分析。结果从人血液中成功扩增出过氧化氢酶基因，获得了重组载体PMD-18T／CAT，氨基酸序列分析的同源性达80％以上。结论从人血液中可以很方便地克隆出过氧化氢酶基因。Objective To clone the catalase gene from blood. Methods The total RNA was isolated from fresh human blood; The eDNA encoding eatalase protein was amplified by reverse tran- scription polymerase chain reaction （RT-PCR） , cloned into the plasmid vector pMD18-T. The ho- mology of amino acids sequence from several catalases was analyzed. Results The catalase gene was cloned successfully from human blood. The recombinant plasmid was established and named pMD18T/CAT. Amino acids sequence alignment indicated that the average value of sequence homology reached to over 80% of homology to catalases. Conclusion The catalase gene in human blood was cloned easily.

关 键 词：人血液 过氧化氢酶基因 克隆 

分 类 号：Q781[生物学—分子生物学]