小麦新抗源CH223抗条锈性的遗传分析及细胞学鉴定  

Inheritance of wheat stripe rust resistance of alien introgression CH223 from Thinopyrum intermedium and its cytological characterization

作　　者：闫金龙[1] 畅志坚[2] 孙美荣[1] 张晓军[2] 詹海仙[2] 李欣[2] 

机构地区：[1]山西大学生物工程学院,太原030006 [2]山西省农业科学院作物遗传研究所,太原030031

出　　处：《植物保护学报》2010年第5期419-424,共6页Journal of Plant Protection

基　　金：国家自然科学基金(30671299;30270710;39870398);山西省回国留学人员科研项目;山西省科技攻关项目(20090311001-2)

摘　　要：用条中（CYR）30、31、32和33号对小麦抗病新品系CH223及其亲本进行抗性评价,分析其对条中32号的抗性遗传方式,并研究其细胞学特征。结果显示,CH223苗期和成株期对上述4个生理小种表现出免疫或近免疫的抗性水平,并具有与其抗性供体TAI7047及其野生亲本中间偃麦草相似的抗病反应型;抗×感的F1代均为免疫,反应型为0~0;型,且F2、F2：3、BC1代的抗、感分离比均符合1对显性基因控制的分离模式;CH223及其与小麦品种＂中国春＂等杂种F1的染色体数目均为2n=42,绝大多数的花粉母细胞具有2n=21Ⅱ的配对构型,并能与小麦染色体完好配对。说明CH223不含较大的外源染色体片段,是一个携带偃麦草抗条锈病基因的隐形异源渐渗系,对条中32号的成株抗性受1对显性核基因控制。Stripe rust caused by Puccinia striiformis f.sp.tritici（Pst） is an important worldwide wheat disease.CH223 is a wheat multi-resistance line developed recently from crossing susceptible wheat cultivars with Thinopyrum intermedium-derived resistant partial amphiploid TAI7047 as donor parents.In this study,the evaluation of resistance reactions demonstrated that CH223 and its donor TAI7047 as well as the wild parents were resistant at the seedling and adult stages to CYR32,the most widely virulent in China,and three other prevalent Pst races,whereas the wheat parents of CH223 and TAI7047 were susceptible,indicating that the stripe rust resistance in CH223 was possibly derived from Th.intermedium.Genetic analysis of the F1,F2,F2：3 and BC1 populations from stripe rust resistant line CH223 revealed that a single dominant gene was responsible for the adult resistance to CYR32.The cytological observation demonstrated that the somatic chromosome number of CH223 and its F1 hybrids involving Chinese Spring wheat was 2n=42,and their most pollen mother cell had a regular pairing of 2n=21Ⅱ at meiosis,suggesting that this resistant line was cytologically a stable cryptic alien introgression,and its chromosome constitution largely identical to that of wheat.

关 键 词：普通小麦 中间偃麦草 新抗源 条锈病 抗性遗传 

分 类 号：S512.1[农业科学—作物学]