4种沼虾的SRFA指纹研究  

Studies on 4 species of Macrobrachium with SRFA fingerprinting

作　　者：邱涛[1] 陆仁后[1] 项超美[1] 张菁[1] 谢浩[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《中国水产科学》1999年第1期1-4,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金;国家九五攻关项目

摘　　要：本文用沼虾属（Ｍａｃｒｏｂｒａｃｈｉｕｍ）的４个主要种：罗氏沼虾（Ｍ．ｒｏｓｅｎｂｅｒｇｉ）、海南沼虾（Ｍ．ｈａｉｎａｎｅｎｓｅ）、日本沼虾（Ｍ．ｎｉｐｐｏｎｅｎｓｅ）、粗糙沼虾（Ｍ．ａｓｐｅｒｕｌｕｍ）作材料，构建了它们的ＳＲＦＡ－ＤＮＡ指纹，经比较得到这４个种的ＳＲＦＡ分子标记，并构建了它们之间的谱系关系图，发现日本沼虾和海南沼虾在遗传上比较接近，属于同一“支序”，粗糙沼虾与另外３者在遗传上都相距较远。This paper demonstrated DNA fingerprinting of 4 species of Macrobrachium (M.rosenbergii,M.hainanense,M.nipponense,M.asperulum)with Selective Restriction Fragment Amplification (SRFA) technique.The similarity of the shared bands amplificated among the 4 species was calculated and the dendrogram of them was drown according to UPGMA method of cluster analysis and the genetic distance data from SRFA.It is found that the genetic distance between M.nipponense and M.hainanense is shorter while those between M.asperulum and other 3 species are longer.

关 键 词：SRFA 沼虾属 指纹 

分 类 号：Q959.223.5[生物学—动物学]