辣椒多聚半乳糖醛酸酶基因CaPG的克隆与序列分析  

Cloning and Sequence Analysis of the Polygalacturonase Gene CaPG in Pepper

作　　者：陈儒钢[1] 杨瑞平[1] 巩振辉[1] 李大伟[1] SHA Hjahan 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2010年第10期1941-1945,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30571262);博士后科学基金(20090451404);博士点基金(20090204120005);陕西省“13115”科技创新工程重大科技专项(2008ZDKG-03);西北农林科技大学“青年学术骨干支持计划”和西北农林科技大学2009年基本科研业务费青年项目资助计划(QN2009010)

摘　　要：以耐贮辣椒品系P98为材料,采用RACE方法,首次获得辣椒果实多聚半乳糖醛酶(PG)基因的全长cD-NA,命名为CaPG,登录号为FJ596175。序列分析结果表明,该基因cDNA长1 668 bp,5′非编码区为119 bp,3′非编码区为442 bp,CDS长1 107 bp,编码368个氨基酸。Blast比对发现,该基因核苷酸序列与已报道的番茄和番木瓜PG基因具有84%和85%的相似性。聚类分析表明,该基因与番茄和番木瓜的亲缘关系较近,与拟南芥PG基因的亲缘关系较远。Pepper（Capsicum annuum L.） line P98 was used as material in this study.A full-length PG cDNA was isolated by rapid amplification of cDNA ends（RACE） and designated as CaPG（GenBank accession number：FJ596175）.The full-length cDNA of CaPG was 1 668 bp long.It includes the putative transcription start site,a potential open reading frame of 1 107 bp,which encodes a 369 amino acid polypeptide,a 5′-untranslated region（5′-UTR） of 119 bp and a 3′-untranslated region（3′-UTR） of 442 bp.Homology analysis of amino acid sequence of CaPG showed an 84% identity with PG-2a in tomato and 85% identity with PG in papaya.Phylogenetic analysis showed that PG gene from pepper and from tomato,papaya was clustered together,and then came those from Arabidopsis.

关 键 词：多聚半乳糖醛酸酶 基因克隆 辣椒 

分 类 号：Q789[生物学—分子生物学] S641.3[农业科学—蔬菜学]