不结球白菜雄蕊心皮化雄性不育系HGMSⅡ特性研究  

Characters of Stamen Carpelloid Male Sterile Line HGMSⅡ of Non-heading Chinese Cabbage

作　　者：张彦锋[1,2] 王学芳[2] 张新[2] 田华丽[1] 王俊生[2] 郭霭光[1] 李殿荣[2] 

机构地区：[1]西北农林科技大学生命科学学院,陕西省分子生物学重点实验室,陕西杨陵712100 [2]陕西省杂交油菜研究中心,陕西大荔715105

出　　处：《西北植物学报》2010年第10期1946-1951,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省分子生物学重点实验室开放课题项目资助(200701)

摘　　要：对不结球白菜雄蕊心皮化雄性不育系HGMS和HGMSⅡ进行花器形态比较、同功酶分析及结实性研究。结果表明:HGMSⅡ较好地保留了HGMS花瓣萼片化、雄蕊心皮化鲜明特征,而且花瓣EST和POD同功酶酶带与萼片酶带基本相同,心皮化器官EST和POD同功酶酶带与雌蕊酶带相同。与完全雄蕊心皮化的HGMS相比,HGMSⅡ雄蕊心皮化突变主要发生在花药部位,导致花药丧失了产生花粉的能力,从而保留了HGMS不育性稳定、彻底的优良特性。与HGMS相比,HGMSⅡ开花习性明显改善,柱头表面花粉明显增多,雌蕊发育基本正常,单株平均籽粒产量达到8.96 g,较HGMS增加了7.17 g,增幅达401%,结实性得到了显著提高。The morphology,isozymes and fecundity of the stamen carpelloid male sterile lines HGMS and HGMSⅡ of non-heading Chinese cabbage were studied.The results showed that HGMSⅡ maintained more petal sepaloid and stamen carpelloid identity of HGMS,and the bands of EST and POD of its petals were same as that of sepals,and the bands of EST and POD of its stamen carpelloid organs same as that of pistils.HGMSⅡ mainly exhibited transformation of anthers into carpels comparing with thorough stamen carpelloid conversion of HGMS,as a result,anther lost ability to generate pollens and exhibited the complete and stable sterility as same as HGMS.Comparing with HGMS,flowering habits of HGMSⅡ got better obviously,and more pollens appeared on stigmas surface,and pistils developed normally,and average seed yield of single plant was 8.96 g,increasing 7.17 g than that of HGMS,the rate of increase reached 401%,so its seed setting improved significantly.

关 键 词：不结球白菜 雄性不育 雄蕊心皮化 

分 类 号：Q945.6[生物学—植物学]