激光扫描共聚焦显微镜在骨髓基质细胞移植中的应用  

Application on transplantation of bone marrow stromal cells with Laser scanning confocal microscope

作　　者：万建美[1] 罗蔚锋[2] 李厚怀[3] 包仕尧[3] 刘春风[2] 张志琳[2] 李冰燕[1] 

机构地区：[1]苏州大学放射医学与辐射防护重点实验室,苏州215123 [2]苏州大学附属第二医院,苏州215007 [3]苏州大学附属第一医院,苏州215007

出　　处：《激光杂志》2010年第5期52-53,共2页Laser Journal

摘　　要：目的:探讨激光扫描共聚焦显微镜(laser scanning confocal microscope,LSCM)技术在骨髓基质细胞(Bone Marrow Stromal Cells,BMSCs)移植中的应用。方法:使用活细胞示踪剂CM-DiI、细胞核探针DAPI和DNA合成前体物BrdU体外标记BMSCs,移植到动物模型体内,利用LSCM技术,观察BMSCs在受体组织内的分布和分化。结果:体外标记BMSCs后再移植到受体,使用LSCM技术可以观察到BMSCs定位于受体心肌组织和脑组织,并有部分BMSCs表达相应的组织特异性蛋白Cx43和GFAP。结论:利用LSCM技术观察BMSCs在受体组织内分布和分化是一种简单可行的方法。Objective：Location and differentiation of bone marrow stromal cells（BMSCs） were investigated with Laser scanning confocal microscope（LSCM）.Methods：After BMSCs in pig and rats were loaded with Cell-Tracker CM-DiI,DAPI and BrdU, they were transplantated into animal model,respectively.Location and differentiation of BMSCs in recipitent was observed with LSCM.Result：Location of BMSCs in recipient tissues was detected.Furthermore,BMSCs could differentiate into myocardial cell or glial cells with expression of Cx43 or GFAP in every group.Conclusion：It is a simple and effective method to observe the Location and differentiation of BMSCs with LSCM.

关 键 词：激光扫描共聚焦显微镜 骨髓基质干细胞 移植 

分 类 号：TN248.1[电子电信—物理电子学]