内吞金纳米粒子的鼻咽癌细胞SERS光谱  

Surfaced-Enhanced Raman Spectroscopic Study on Single Living Human Nasopharyngeal Carcinoma Cells Incubated with Colloidal Gold

作　　者：黄浩[1] 潘建基[2] 陈伟炜[1,3] 陈奇松[2] 冯尚源[3] 苏颖[2] 许雄伟[4] 陈荣[3] 

机构地区：[1]福建中医药大学中西医结合学院,福建福州350108 [2]福建省肿瘤医院,福建福州350014 [3]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007 [4]福建医科大学附属第二医院,福建泉州362000

出　　处：《光谱学与光谱分析》2010年第11期2981-2984,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60778046,60910106016);福建省科技项目(2008I0015,2008J0016);卫生部科研基金项目(WKJ2008-2-046)资助

摘　　要：采用内吞方法将金纳米粒子引入细胞内,测试分析单个活性CNE-1鼻咽癌细胞的常规拉曼光谱和SERS光谱,并对其进行初步谱峰归属。CNE-1细胞的常规拉曼光谱有6个主要的拉曼峰:718,1001,1123,1336,1446和1660cm-1;沉积于细胞内的金纳米粒子强烈地增强了细胞内生化物质拉曼信号,在内吞金纳米粒子的CNE-1细胞的拉曼光谱中出现了20多个SERS拉曼信号,主要拉曼峰的强度明显高于常规拉曼信号。DNA骨架振动(1026,1097,1336和1585cm-1)证明金纳米粒子通过内吞作用而进入细胞核内。结果表明,基于胶体金SERS技术可能为活性鼻咽癌细胞内生化物质的探测提供一种高灵敏的方法。The surface-enhanced Raman scattering（SERS）spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells（CNE-1）were tested and analyzed by gold nanoparticles incubation into cells.Six obvious Raman bands（718,1 001,1 123,1 336,1 446 and 1 660cm-1）were observed in the normal Raman spectroscopy of living CNE-1 cells.The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned.Colloidal gold particles that were introduced inside cells result in strongly enhanced Raman signals of the native chemical constituents of the cells,and over twenty SERS Raman bands were observed in the SERS spectroscopy of living CNE-1 cells.The Raman lines of 1 026,1 097,1 336 and 1 585cm-1 were assigned to vibrations of the DNA backbone,which confirms that some gold nanoparticles were able to enter the nucleus.The results showed that,based on colloidal gold,the SERS spectroscopy might provide a sensitive and structurally selective detecting method for native chemicals inside a cell,such as DNA and phenylalanine.

关 键 词：表面增强拉曼散射(SERS) 金溶胶 人鼻咽癌细胞株 

分 类 号：O657.3[理学—分析化学] R739.6[理学—化学]