大孔吸附树脂法富集纯化荆芥穗总黄酮的工艺研究  

Study on the Enrichment and Purification of Total Flavonoids inSchizonepeta tenuifolia by Macroporous Adsorption Resin

作　　者：黄志宏[1,2] 蒋东旭[1,2] 赖小平[1,2] 

机构地区：[1]广州中医药大学新药开发研究中心,广东广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中药材》2010年第9期1476-1480,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:对大孔树脂富集纯化荆芥穗总黄酮的工艺条件及参数进行优化。方法:先以总黄酮比吸附量和解吸率为指标,通过静态吸附与解吸试验对9种树脂型号进行筛选,再通过动态吸附与解吸试验对富集纯化工艺参数进行全面优化。结果:HP-20树脂对荆芥穗总黄酮的吸附与解吸性能较好;确定最佳吸附条件为径高比1∶6,样品溶液pH值为6.0,浓度0.4 g(生药)/mL,吸附流速2 BV/h和最佳吸附量133.76 mg(总黄酮)/g(干树脂);最佳解吸条件为80%乙醇洗脱,洗脱流速4 BV/h和溶剂用量3 BV。结论:HP-20树脂可有效地对荆芥穗总黄酮进行富集纯化。Objective：To establish an optimum enrichment and purification process of total flavonoids in Schizonepeta tenuifolia by macroporous resins.Methods：9 kinds of resins were compared and the best one was chosen.Then the technique condition for separating and purifying total flavonoids from Schizonepeta tenuifolia was studied.Results：HP-20 was selected for its excellent adsorption and desorption properties,80% ethanol was found to be the best elution solution.The best result was based on the follows： pH value-6.0,feeding rate-2 BV/h,sample concentration-0.4 g/mL,the ratio of total flavonoids to HP-20 macroporous resin-133.76 mg/g,elution flowing rate-4 BV/h,eluting agent amount-3 BV.Then the purity reached 74.31% and the yield was 97.13%.Conclusion：The total flavonoids of Schizonepeta tenuifolia can be effectively purificated and separated by HP-20 macroporous resin.

关 键 词：大孔树脂 荆芥穗 总黄酮 富集纯化 

分 类 号：R284.2[医药卫生—中药学]