Simultaneous determination of metronidazole and tinidazole in plasma by using HPLC-DAD coupled with second-order calibration  

作　　者：Li Qun Ouyang Hai Long Wu Ya Juan Liu Jian Yue Wang Yong Jie Yu Hong Yan Zou Ru Qin Yu 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China [2]Zhoushan Municipal Center for Disease Control & Prevention, Zhoushan 310062, China

出　　处：《Chinese Chemical Letters》2010年第10期1223-1226,共4页中国化学快报（英文版）

基　　金：financially supported by The National Natural Science Foundation of China(No.20775025);The National Basic Research Program(No.2007CB216404) as well as PCSIRT

摘　　要：A method using HPLC-DAD coupled with second-order calibration was developed to simultaneously determine metronidazole and tinidazole in plasma samples in this paper. The second-order calibration method based on APTLD （alternating penalty trilinear decomposition） algorithm was proposed to analyze the three-way HPLC-DAD data from both standard and prediction samples, which makes it possible that calibration can be performed even in the presence of unknown interferences with a simple and green chromatographic condition and short analysis time. The results showed that good recoveries were obtained although the chromatographic and spectral profiles of the analytes of interest as well as background were partially overlapped with each other in plasma samples.A method using HPLC-DAD coupled with second-order calibration was developed to simultaneously determine metronidazole and tinidazole in plasma samples in this paper. The second-order calibration method based on APTLD （alternating penalty trilinear decomposition） algorithm was proposed to analyze the three-way HPLC-DAD data from both standard and prediction samples, which makes it possible that calibration can be performed even in the presence of unknown interferences with a simple and green chromatographic condition and short analysis time. The results showed that good recoveries were obtained although the chromatographic and spectral profiles of the analytes of interest as well as background were partially overlapped with each other in plasma samples.

关 键 词：HPLC-DAD Second-order calibration Alternating penalty trilinear decomposition （APTLD） METRONIDAZOLE TINIDAZOLE PLASMA 

分 类 号：O657.72[理学—分析化学] O657.32[理学—化学]