Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection  

作　　者：Yin Yan Zhang Zi Cheng Li Jin Kun Zhu Zhi Yong Yang Qing Jiang Wang Pin Gang He Yu Zhi Fang 

机构地区：[1]Department of Chemistry, East China Normal University, Shanghai 200062, China

出　　处：《Chinese Chemical Letters》2010年第10期1231-1234,共4页中国化学快报（英文版）

基　　金：financially supported by The Program for New Century Excellent Talents in University

摘　　要：A high-performance capillary electrophoresis with amperometry detection method （CE-AD） has been developed for the analysis of flavonoids and anthraquinones （emodin, kaempferol, apigenin, luteolin and rhein） in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer （1.5 ×10^-2 mol/L borate-3× 10^-2 mol/L phosphate running buffer, pH 9.0） at a working potential of ＋0.90 V （vs. SCE） and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits （S/N = 3） ranging from 1.0 × 10^-7 to 2.1 × 10^-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.A high-performance capillary electrophoresis with amperometry detection method （CE-AD） has been developed for the analysis of flavonoids and anthraquinones （emodin, kaempferol, apigenin, luteolin and rhein） in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer （1.5 ×10^-2 mol/L borate-3× 10^-2 mol/L phosphate running buffer, pH 9.0） at a working potential of ＋0.90 V （vs. SCE） and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits （S/N = 3） ranging from 1.0 × 10^-7 to 2.1 × 10^-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

关 键 词：Capillary electrophoresis Amperometry detection Chrysanthemu FLAVONOIDS ANTHRAQUINONES 

分 类 号：O657.8[理学—分析化学] TS252.51[理学—化学]