Effects of a Biosurfactant and a Synthetic Surfactant on Phenanthrene Degradation by a Sphingomonas Strain  

作　　者：PEI Xiao-Hong ZHAN Xin-Hua WANG Shi-Mei LIN Yu-Suo ZHOU Li-Xiang 

机构地区：[1]College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China) [2]Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing 210042 (China)

出　　处：《Pedosphere》2010年第6期771-779,共9页土壤圈（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 20377024);the National High Technology Research and Development Program (863 Program) of China (Nos. 2007AA061101 and 2009AA063103)

摘　　要：A novel phenanthrene (PHE)-degrading strain named as Sphingomonas sp.GF2B was isolated and identified from a farmland soil.Effects of a synthetic surfactant (Tween-80) and a rhamnolipid biosurfactant on PHE degradation by Sphin- gomonas sp.GF2B were investigated at different concentrations of the surfactants.The results showed that Sphingomonas sp.GF2B was able to mineralize up to 83.6% of PHE within 10 days without addition of surfactants.The addition of Tween-80 to the reaction medium inhibited greatly PHE biodegradation,with only 33.5% of PHE degraded.However,the biosurfactant facilitated PHE biodegradation,with up to 99.5% of PHE degraded.The preferential utilization of PHE as a carbon source and the enhanced solubility of PHE by the biosurfactant were likely responsible for the higher biodegra- dation efficiency of PHE in the presence of the biosurfactant.Therefore,it could be concluded that the application of the biosurfactant to PHE-contaminated soils was perhaps a feasible way to facilitate the PHE biodegradation.A novel phenanthrene （PHE）-degrading strain named as Sphingomonas sp. GF2B was isolated and identified from a farmland soil. Effects of a synthetic surfactant （Tween-80） and a rhamnolipid biosurfactant on PHE degradation by Sphingomonas sp. GF2B were investigated at different concentrations of the surfactants. The results showed that Sphingomonas sp. GF2B was able to mineralize up to 83.6% of PHE within 10 days without addition of surfactants. The addition of Tween-80 to the reaction medium inhibited greatly PHE biodegradation, with only 33.5% of PHE degraded. However, the biosurfactant facilitated PHE biodegradation. with up to 99.5% of PHE degraded. The preferential utilization of PHE as a carbon source and the enhanced solubility of PHE by the biosurfactant were likely responsible for the higher biodegra- dation efficiency of PHE in the presence of the biosurfactant. Therefore, it could be concluded that the application of the biosurfactant to PHE-contaminated soils was perhaps a feasible way to facilitate the PHE biodegradation.

关 键 词：biodegradation contaminated soils RHAMNOLIPID solubility TWEEN-80 

分 类 号：TQ423[化学工程] O629.11[理学—有机化学]