心宁片对麻醉犬急性心肌缺血的保护作用  

Protective Effect of Xinning Tablet on Acute Myocardial Ischemia in Anaeshetic Dogs

作　　者：蔡光先[1] 王宇红[2] 

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学第一附属医院中药药理(心血管)实验室,长沙410007

出　　处：《世界科学技术-中医药现代化》2010年第5期752-758,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖南省科技厅重点项目(05SK2007):治疗冠心病五类新药心宁缓释片的研制;负责人:蔡光先

摘　　要：目的:观察心宁片对实验性心肌缺血的保护作用。方法:结扎犬冠状动脉前降支制备犬急性心肌梗死模型;超声多普勒方法测定心肌血流量;用心外膜电图描记和硝基蓝四唑(N-BT)组织化学染色方法计算心肌缺血程度及心肌梗死面积;放射免疫法测定血浆内皮素(ET)和血栓烷素B_2(TXB_2)的含量。结果:心宁片1.2、0.6g·kg^(-1),于药后30～180min能明显减轻由心外膜电图标测的犬心肌缺血程度:缩小经NBT染色所显示的梗死区;于药后30～120min显著增加缺血心脏的心肌血流量:对心肌缺血及心肌梗死引起的血浆ET和TXB_2的含量升高有明显的抑制作用,升高心肌缺血时血浆6-Keto-PGF_(1α)和6-Keto-PGF_(1α)/TXB_2的比值。结论:心宁片对犬急性心肌缺血和心肌梗死有明显的保护作用,其作用机制可能与抑制ET、TXA_2的释放,升高6-Keto-PGF_(1α)含量,增加心肌血流量,从而改善受损心脏的功能有关。This work aimed to observe the protective effect of Xinning tablets on experimental myocardial ischemia. The canine model of acute myocardial infarction was established by ligating the anterior descending coronary artery of dogs. The ultrasound Doppler method was adopted to determine myocardial blood flow. Heart membrane electrical diagram coup and nitro blue tetrazolium （N-BT） staining were used to calculate the extent of myocardial ischemia and myocardial infarct size. Radioimmunoassay was used to determine plasma ET, TXB2 and 6-Keto-PGF1α levels. 30-180 min after administration of Xinning tablets at 1.2 and 0.6 g/kg, the dog myocardial ischemia icon measured from the epicardial electrogram was significantly reduced; the infarct area was decreased according to NBT staining. 30-120 after the administration, cardiac blood flow was significantly increased in the myocardial ischemia area, the levels of plasma ET and TXB2 were greately inhibited, and the 6-Keto-PGF1α content to TXB2 / 6-Keto-PGF1α ratio was increased. Taken together, Xinning tablets have significant protective effect on dog acute myocardial ischemia and myocardial infarction, the mechanism of which may be related to ET inhibition, TXA2 release and increased myocardial blood flow, consequently improving the impaired heart function.

关 键 词：心宁片 心肌缺血 心肌血流量 心外膜电图 内皮素 血栓烷素B26-酮-前列 腺素F1α 

分 类 号：R542.2[医药卫生—心血管疾病]