“永生源”铁皮石斛中多糖含量测定方法的比较研究  

Comparative Studies on Determination of Polysacharide in Dendrobium officinale

作　　者：刘宏源 杨金燕 黄松[2] 黄斌[2] 赖小平[2] 

机构地区：[1]广东永生源生物科技有限公司,饶平515736 [2]广州中医药大学新药研究与开发中心,广州510006

出　　处：《世界科学技术-中医药现代化》2010年第5期798-801,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广东省科技厅中药现代化专项(K5080016):砂仁等10种岭南药材规范化种植(养殖)关键技术研究;负责人:徐鸿华

摘　　要：目的:筛选铁皮石斛中多糖含量测定的最佳方法。方法:分别采用苯酚-浓硫酸比色法和3,5-二硝基水杨酸比色法(DNS法)测定铁皮石斛中多糖的含量。结果:苯酚-浓硫酸法测定铁皮石斛中多糖含量偏高。结论:DNS法较为适合铁皮石斛中多糖含量测定,且所建立的方法操作简便、稳定、可重复,可用于铁皮石斛药材及其多糖类保健品的质量控制。This work aimed to select a better assay method for polysacharide in Dendrobium officinale. Phenol-sulfuric acid colorimetry and 3,5-dinitrosalicylic acid colorimetry （DNS） were respectively adopted to determine watersoluble polysaccharide. The content of polysaccharide determined by phenol-sulfuric acid colorimetry was higher than by 3, 5-dinitrosalicylic acid colorimetry. The results showed that 3, 5-dinitrosalicylic acid colorimetry is more suitable for the determination of polysacharide in D. officinale. This method has been proven to be simple, stable and repeatable, thus may be used for the quality control of crude D. officinale and medical care preparations containing polysaccharide extracted from D. officinale.

关 键 词：铁皮石斛 多糖苯酚-浓硫酸比色法3 5-二硝基水杨酸比色法 含量测定 

分 类 号：R282.71[医药卫生—中药学]