CD1_D基因与B7-1基因真核表达载体的构建  

Construction of recombinant eukaryotic vectors expressing CD1_D gene and B7-1 gene

作　　者：王昆华[1] 张杰[2] 龚昆梅[1] 肖乐[1] 欧阳一鸣[1] 刘为军[1] 凌平[1] 黄映光[1] 龙亚新[1] 郭世奎[1] 

机构地区：[1]云南省第一人民医院普通外科,云南昆明650032 [2]云南省第一人民医院遗传诊断中心

出　　处：《中国肿瘤外科杂志》2010年第4期199-201,206,共4页Chinese Journal of Surgical Oncology

摘　　要：目的构建表达小鼠CD1_D基因和B7-1基因的真核表达载体。方法用PCR方法获得基因,定向克隆连接到真核表达载体上,得到重组真核表达载体,使用酶切方法加以鉴定。结果酶切得到的片段与所需相符,得到重组的逆转录病毒载体。结论重组小鼠CD1_D基因和B7-1基因真核表达载体的构建为今后进一步的研究奠定了基础。Objective To construct recombinant eukaryotic vectors expressing B7-1 gene and CD1D gene. Methods PCR was performed in order to get BT-1 gene and CD1D gene, then they were ligated into eukaryotic vectors, and identified by digestion. Results The amplified fragments were coincident to tile goal product. Recombinant cukaryotic vectors were obtained. Conclusions We got recombinant eukaryotic vectors expressing B7-1 gene and CD1D gene which provides a foundation for the further study.

关 键 词：CD1D基因 B7—1基因 真核表达载体 RNA提取 重组载体的构建 

分 类 号：R341[医药卫生—基础医学]