血塞通体内中和内毒素作用的试验研究  

Neutralizing endotoxin effect of Xuesaitong injection

作　　者：穆德广[1] 傅恩清[1] 金发光[1] 谢永宏[1] 南岩东[1] 

机构地区：[1]第四军医大学唐都医院呼吸内科,陕西西安710038

出　　处：《现代生物医学进展》2010年第19期3632-3634,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨血塞通体内中和内毒素的作用。方法:健康大耳白兔24只,随机分为正常对照组、内毒素组、血塞通干预组,每组8只。内毒素组经耳缘静脉静推LPS(1mg/kg),12h后再由耳缘静脉注射一次LPS(1mg/kg),复制SIRS/MODS动物模型;对照组耳缘静脉静推等量生理盐水;血塞通干预组于0h,12h经耳缘静脉推注LPS(1mg/kg)并于第一次注入内毒素后6h,12h,18h静推血塞通(50mg/kg)。于24h时间点测定各组动物血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-8(IL-8)、血小板活化因子(PAF)。光镜下观察各组动物脏器病理学变化。结果:血塞通干预组与内毒素组相比,IL-1、IL-8、PAF、TNF-α均较内毒素组有不同程度降低。血塞通干预组脏器损伤组织学改变较内毒素组明显减轻。结论:血塞通在体内能明显中和内毒素的作用,为防治SIRS/MODS提供了实验依据。Objective： To observe the neutralizing endotoxin effect of Xuesaitong injection. Methods： 24 rabbits were randomly divided into three groups： control group （n = 8）, injury group （n = 8）, treatment group （n=8）. The injury group were given endotoxin （1mg/kg） at the 0, 12 h time points; the control group were given injection of normal saline （1mg/kg） instead of the endotoxin; and the treatment group were given Xuesaitong injection （50mg/kg） 6, 12, 18 h time points besides the twice injection of endotoxin （1mg/kg） at the 0, 12 h time points; TNF- α, IL-1, IL-8, PAF concentration were measured at the 24 h time point. The pathological changes were observed under microscope. Results： TNF-α, IL-1, IL-8, PAF concentrations of the treatment group were significant different from those of control group and injury group. Pathology showed Xuesaitong injection intervention group reduced lung and kidney injury. Conclusion： Xuesaitong injection has neutralizing endotoxin effect against SIRS/MODS.

关 键 词：SIRS/MODS 血塞通 内毒素 

分 类 号：R453[医药卫生—治疗学]