不同方法检测血清HBV DNA水平的比较  

Comparison of different methods to detect the HBV DNA level in serum

作　　者：丁世涛[1] 汤影子[1] 范熠[1] 张欣欣[2] 况雪梅[1] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,重庆400038 [2]上海交通大学医学院附属瑞金医院,上海200255

出　　处：《现代生物医学进展》2010年第19期3658-3659,共2页Progress in Modern Biomedicine

摘　　要：目的:研究不同方法检测血清HBVDNA水平的比较。方法:采用151份标本分别采用COBAS Amplicor、实时定量PCR(LightCycler及Mx3000p)方法进行平行检测。结果:①检测灵敏度:COBAS Amplicor方法灵敏度高于实时PCR方法(P=0.005、0.014),而Mx3000p灵敏度高于LightCycler方法(P=0.042)。②相关性:实时定量PCR(LightCycler及Mx3000p)与COBAS Amplicor方法所测HBVDNA结果均呈线性相关(r=0.842,P<0.01;r=0.946,P<0.01)。结论:实时定量PCR方法是较为经济且准确的HBVDNA检测方法,而Mx3000p在准确性和灵敏度上可能优于LightCycler方法。Objective： To compare the results of detecting HBV DNA level in serum by different methods. Methods： A total of 151 Samples were selected and detected separately by COBAS Amplicor,Real-Time PCR （LightCycler and Mx3000p）. Results： ① Detection of sensitivity：COBAS Amplicoris was higher than Real-Time PCR （P=0.005,0.014）, while Mx3000p ,higher than LightCycler （P=0.042） ② Correlation： The results of Real-Time PCR （LightCycler and Mx3000p） and COBAS Amplicor all showed a linear correlation （r=0.842,P〈0.01;r= 0.946,P〈0.01）. Conclusion： Real-Time PCR is an economical and accurate method to detect HBV DNA in the serum,and Mx3000p is superior to LightCycler in accuracy and sensitivity.

关 键 词：HBV DNA 荧光定量PCR COBAS AMPLICOR 

分 类 号：R446[医药卫生—诊断学]