固本颗粒胶囊对大鼠COPD模型血清IL-8及TNF-α影响的实验研究  

The Effect of GuBenKeLi Capsule on the Contents of Cytokines in Serum of COPD Rats Model

作　　者：王平丽[1] 周兆山 胡海波 杨晓萍[1] 殷彬[3] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛市海慈医疗集团,山东青岛266033 [3]山东中医药大学,山东青岛266033

出　　处：《现代生物医学进展》2010年第19期3663-3665,3687,共4页Progress in Modern Biomedicine

基　　金：青岛市科技发展计划(No05-2-NS41)

摘　　要：目的:根据COPD慢性气道炎症学说,探讨固本颗粒胶囊对COPD模型大鼠血清中相关细胞因子的影响,从而阐明其防治COPD的作用机理,为固本颗粒胶囊的临床应用提供实验依据。方法:将50只Wistar大鼠随机分成正常对照组、模型对照组、强的松组、固本颗粒胶囊低剂量组、固本颗粒胶囊高剂量组,每组10只,以烟熏及气管内滴注脂多糖的方式建立大鼠COPD模型,予相应药物干预,观察记录大鼠一般状况,至实验结束,酶联免疫吸附法检测大鼠血清中相关细胞因子(IL-8、TNF-α)的含量。相关实验数据用SPSS10.0统计软件进行统计学处理。结果:大鼠一般状况及组织病理学改变提示COPD模型复制成功,与正常对照组比较,模型对照组大鼠血清中相关细胞因子(IL-8、TNF-α)的含量水平失衡。在改善COPD模型大鼠的一般状况、病理学改变及细胞因子含量比例失衡的作用结果来看,各治疗组与模型对照组均有显著差异(p<0.05),综合比较,强的松组治疗作用最为显著,固本颗粒高剂量次之,固本颗粒低剂量组作用最弱。结论:①.固本颗粒胶囊能有效缓解COPD模型大鼠临床表现及气道炎症,其作用机制在于:能纠正COPD大鼠体内细胞因子水平失衡;②.上述作用,具有一定的量效关系,即随着给药剂量的增加有加强趋势。Objective： To investigate the effects of GuBenKeLi Capsule on the contents of cytokines （IL-8, TNF-α） in serum of COPD Rats Model and explore the mechanism of treating COPD with GuBenKeLi capsule. Methods： 50 male Wistar rats were divided randomly into five groups： Normal control group, Model control group, Prednisone group, GuBenKeLi capsule low dose group, GuBenKeLi capsule high dose group, each group with 10 rats. Then we copied COPD Rats Model by being smoked and inhaled with LPS. At the same time, each group was given relevant medicines. After eight weeks, we killed all the rats. And then we surveyed the contents of cytokines （IL-8, TNF-α）in serum. Results： Changes of bronchial and pulmonary histopathology in each group were typical, pointed out that COPD Rats Model were successful. Compared to Normal control group, Model control group had unbalance in the contents of cytokines in serum. Compared comprehensively, D Prednisone group were better than other groups, GuBenKeLi capsule high dose group secondly, GuBenKeLi capsule low dose group worstly. Conclusion： GuBenKeLi capsule could alleviate the airway inflammation and affected the contents of cytokines in serum. Above-mentioned action can be reinforced by increased dose.

关 键 词：固本颗粒胶囊 COPD模型大鼠 IL-8 TNF-Α 

分 类 号：Q95-3[生物学—动物学] R563.3[医药卫生—呼吸系统]