Optimization of Biosurfactant Production from Glycerol by Pseudomonas Aeruginosa EQ 109 Using Factorial Design 2^3  

作　　者：Francisca Pessoa De Franca Flavia Padilha Bastos Dos Santos Lea Maria de Almeida Lopes 

机构地区：[1]School of Chemistry, Federal University of Rio de Janeiro, CT, BI. E, Fundao Island, 21949-909, Rio de Janeiro, RJ, Brazil [2]Institute of Maeromolecules Professora Eloisa Mano /Lapin, Federal University of Rio de Janeiro, CT, Bl. J, Fundao Island, 21941-598. Rio de Janeiro. RJ. Brazil

出　　处：《Journal of Chemistry and Chemical Engineering》2010年第10期1-12,共12页化学与化工（英文版）

摘　　要：One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant production from glycerol by Pseudomonas aeruginosa EQ 109 isolated from crude oil-contaminated soil. Factorial design 2^3 was utilized to optimize the amount of biosurfactant produced, by using pH （A）, initial biomass concentration （B）, and initial glycerol concentration （C） as independent factors. The experiments were carried out in flasks containing 100 mL of mineral medium. Biosurfactant production was monitored by increase of the emulsification of aviation kerosene （E24） and surface tension reduction （STr）. The results have shown that, at pH = 7.0, in order to increase E24, variables as B and C are the most influential, leading to a maximum value of E24 = 79%, as well as for an increase of GC （GCmax = 49%）. STR was the variable with the best correlation factor for the proposed linear model （R2=0.96） and its maximum value was 48%. Xfwas not significant to the model, although it was influenced by pH and C, with C = 40g/L （Xfmax = 4.56 g/L）.

关 键 词：BIODIESEL BIOSURFACTANT factorial design GLYCEROL Pseudomonas aeruginosa. 

分 类 号：S858.315.1[农业科学—临床兽医学] TQ423[农业科学—兽医学]