毛细管区带电泳同时检测人尿和头发中6种毒品  

Simultaneous Determination of Drugs of Abuse in Human Urine and Hair by Capillary Zone Electrophoresis with Ultraviolet Detection

作　　者：孟梁[1] 臧祥日[1] 申贵隽[1] 王正巧[2] 王莉莉[1] 祝玲[1] 

机构地区：[1]大连大学环境与化学工程学院,大连116622 [2]大连市公安局刑事科学技术研究所,大连116030

出　　处：《分析化学》2010年第10期1474-1478,共5页Chinese Journal of Analytical Chemistry

基　　金：公安部应用创新计划(No.2007YYCXLNST021)资助项目

摘　　要：建立了一种毛细管区带电泳紫外法同时分离检测人尿和头发中的阿片类、苯丙胺类及氯胺酮等6种毒品的新方法。在100mmol/L磷酸盐缓冲溶液(pH5.5),电迁移进样和分离电压13kV的条件下,在8min内实现了6种毒品的分离检测。在优化条件下,各药物在0.05～200mg/L范围内存在良好的线性关系,r为0.9982～0.9992;检出限为16～30μg/L;RSD<2.5%(n=5)。本方法成功应于人尿和头发样品中毒品的检测,其回收率为95%～103%;检出限分别为20～35μg/L和0.2～0.4μg/g;RSD<4.7%(n=5)。A new method was developed for the simultaneous determination of multiple drugs of abuse in human urine and hair using capillary electrophoresis with UV detection （CE-UV） .Parameters affecting resolution,such as the pH and the concentration of electrolyte solution,the running voltage,were investigated and optimized.Under optimum conditions,linearity of the method was observed for all target analytes in the range from 0.05 to 200 mg/L with the correlation coefficient （R2） ranging from 0.9982 to 0.9992.The limits of detection （LODs,S/N=3） was estimated to be in the range from 16 to 30 μg/L.Excellent repeatability （RSD ≤2.5%,n =5） was achieved.The feasibility of this method was demonstrated by analyzing human urine and hair samples with spiked recoveries in the range of 95%-103% .Also,the evaluation of linearity and analytical accuracy of the method provided good results for all the analytes investigated,thus allowing its application to real cases of seized controlled drugs determination.

关 键 词：毛细管区带电泳 阿片类毒品 苯丙胺类毒品 氯胺酮 尿 头发 

分 类 号：D919.4[医药卫生—法医学]