稀土离子络合物荧光探针测量胆红素及其荧光猝灭机理研究  

Determination of Bilirubin by Fluorescent Probe of Rare Earth Ion Complex and Discussion of Fluorescence Quenching Mechanism

作　　者：边玮玮[1] 张娜[2] 阎芳[1] 王守训[1] 李耀辉[1] 

机构地区：[1]潍坊医学院医用化学教研室,潍坊261053 [2]黄山学院化学系,黄山245041

出　　处：《分析化学》2010年第10期1501-1504,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30572323);潍坊医学院科研资金(No.KQ2007030)资助项目

摘　　要：在NH4Ac-HAc缓冲溶液(pH6.90)中,强力霉素可以和铕离子(Eu3+)生成二元络合物,能量从强力霉素转移到Eu3+,发射出Eu3+位于612nm处的特征荧光。加入胆红素后,强力霉素将能量转移给胆红素。因此,Eu3+位于612nm处的特征荧光强度降低,且降低的荧光强度与加入胆红素的浓度成正比,据此建立了荧光光度法测量胆红素的方法。在最佳实验条件下,测量的线性范围为5.0×10-8～3.0×10-5mol/L;检出限为8.4×10-9mol/L。本方法操作简单,可以较好地避免共存物质的干扰,并成功用于人血清样品中胆红素含量的测定。In NH4Ac-HAc buffer solution （pH 6.90） ,doxycycline（DC） can form a binary complex with Eu3＋ ,in which DC can transfer energy to Eu3 ＋ and emit the characteristic peak of Eu3 ＋ at λem=612 nm.After adding bilirubin,the fluorescence intensity at λem=612 nm of DC-Eu3 ＋ was decreased and the decreased fluorescence intensity was proportional to the concentration of bilirubin.Based on the phenomena,a new spectrofluorimetric method for determination of bilirubin was developed.Under the optimal experimental conditions,the linear range and detection limit were 5.0 × 10-8-3.0 × 10-5 mol/L and 8.4 × 10-9 mol/L respectively.This method is simple,practical and relatively free of interference from coexisting substances.The method has been successfully applied to determine bilirubin in serum samples.

关 键 词：强力霉素 铕 血清 胆红素 荧光 

分 类 号：R341[医药卫生—基础医学]