大孔树脂纯化八珍汤苷类部位的研究  

Study on purification of glycosides from Bazhen decoction with macroporous resin

作　　者：丁振铎[1] 桑咏梅[1] 薛佳[2] 胡杨[2] 李文兰[2] 

机构地区：[1]黑龙江生态工程职业学院,黑龙江哈尔滨150025 [2]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2010年第5期518-523,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江博士后科研启动资助金项目

摘　　要：采用大孔吸附树脂技术对苷类部位进行纯化.以芍药苷、人参皂苷Rb1及总苷(以芍药苷计)、总皂苷(以人参皂苷Re计)的含量为指标,筛选了8种大孔树脂,优选出吸附容量及洗脱效果较好的D-101型大孔树脂,通过对吸附动力学曲线、药液pH值、漏点质量浓度的考察,确定最佳吸附条件为药液质量浓度0.25 g/mL,药液pH值为6,吸附时间为8 h;通过对洗脱液质量分数、洗脱液pH值、洗脱液体积、洗脱液流速的考察,确定最佳洗脱条件为洗脱液质量分数为80%的乙醇,pH值为8,洗脱液体积为40 BV,洗脱流速为3 BV/min.芍药苷、人参皂苷Rb1及总苷(以芍药苷计)、总皂苷(以人参皂苷Re计)的洗脱率分别为98.14%、96.83%、91.71%、91.21%,终产品中总苷类成分的百分含量占总洗脱物的24.44%、总皂苷类成分的百分含量占总洗脱物的68.90%.Absorption characteristic of eight macroporous adsorption resins for element of total glycosides and total saponins in Bazhen decotion is investigated.D-101 macroporous resin,which has the best absorption capacity,is chosen to do separation and purification research.By absorption kinetics curve,the effect of pH and the leakage concentration,the conclusion is that the leakage concentration is 0.25 g/mL,pH is 6 and the extracted solution is absorbed for 8 hour.By the effect of different concentration,pH value,volume and flow velocity of eluent,the result shows that eluted concentration is 80% ethanol,pH is 8,eluted volume is 40 times amount and the velocity of eluent is 3 BV/mL.The eluting rate of paeoniflorin,ginsenoside,total glycosides and total saponins are 98.14%,96.837%,91.71%,91.21%.The result shows that D-101 resin can adsorb and desorb these components well.The purity of total glycosides is 24.44% and total saponins is 68.90%.

关 键 词：大孔吸附树脂 八珍汤 总苷 芍药苷 人参皂苷RB1 

分 类 号：R284[医药卫生—中药学]