丙型肝炎病毒感染及其抗原表达与细胞凋亡的关系  

The Relationships Between HCV Infection, Expression of HCV Antigens in Tissues and Apoptosis

作　　者：彭晓谋[1] 彭文伟[1] 姚春斓[1] 陈雪娟[1] 顾琳[1] 

机构地区：[1]中山医科大学附属第三医院病毒性肝炎研究室

出　　处：《中山医科大学学报》1999年第2期111-114,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：美国中华医学基金会(CMB)资助

摘　　要：目的：细胞模型上研究表明，ＨＣＶ抗原能抑制细胞凋亡。本研究在体内研究水平进一步探讨ＨＣＶ抗原表达对细胞凋亡的影响。方法：采用原位细胞凋亡、免疫组织化学及其双染色等技术，对７０例肝细胞癌（ＨＣＣ）和１０例慢性肝炎的组织标本进行研究。结果：ＨＣＶ感染或组织ＨＣＶ抗原表达阳性的患者中，细胞凋亡指数与阴性患者无显著性差异。而仅有纤维化的慢性肝炎组织较伴有肝硬变的癌周组织的凋亡指数显著升高（２０２７±２４１ｖｓ８４７±３２４，Ｐ＜００１）。在分布上，慢性肝炎组织中细胞凋亡与抗原分布一致，与炎症反应密切相关，而癌周组织中两者常不一致。结论：在组织水平上ＨＣＶ抗原表达对细胞凋亡的影响相对复杂。感染早期，ＨＣＶ抗原主要通过炎症反应而促进细胞凋亡。Objective: HCV antigens can inhibit apoptosis in vitro on cell model. The impact of expression of HCV antigens on apoptosis in tissue level was probed into in the research. Methods: Expression of HCV antigens and apoptosis were demonstrated using IHC and in situ cell death detecting in samples from 70 HCC and 10 chronic hepatitis. Results: Apoptotic index of the group with HCV infection or expression of HCV antigens in liver tissues was not significantly higher than that of the control group. Apoptotic index of chronic hepatitis, however, was significantly higher than that of HCC patients with cirrhosis in pericancerous tissues (20 27±2 41 vx 8 47±3 24, P <0 01). The distribution of apoptotic cell and expression of HCV antigens was consistent, was both strongly related to inflammation. The distribution, however,in pericancerous tissues was irrelevant. Conclusions: These results suggested that HCV antigens had more complex affections on apoptosis in tissue level than in vitro. HCV antigens maingly increased apoptosis through inflammation in the early stage of infection and inhibited apoptosis in the later stage.

关 键 词：丙型肝炎 HCV 感染 抗原表达 细胞凋亡 免疫学 

分 类 号：R512.63[医药卫生—内科学]