巨噬细胞条件培养基促进许旺细胞分泌神经生长因子的实验研究  

Experimental study on the macrophages conditioned media(MΦCM)influence NGF secretion in adult rat schwann cells in vitro

作　　者：马向阳[1,2] 于涯涛 钟世镇[1,2] 胡耀民[1,2] 王国英 陈立龙[1,2] 

机构地区：[1]第一军医大学解剖学教研室 [2]中国人民解放军第157医院骨科

出　　处：《中华显微外科杂志》1999年第2期120-121,共2页Chinese Journal of Microsurgery

基　　金：国家自然科学基金

摘　　要：目的研究巨噬细胞对许旺细胞分泌神经生长因子（ＮＧＦ）的影响。方法制备成年大鼠腹腔巨噬细胞的几种条件培养基，分别施加于纯化培养的成鼠许旺细胞作用２４小时，取细胞上清液，用间接ＥＬＩＳＡ法测定细胞上清液中ＮＧＦ的含量。结果变性髓鞘及脂多糖激活的巨噬细胞条件培养基可以明显促进许旺细胞分泌ＮＧＦ。结论巨噬细胞条件培养基中含有可以促进许旺细胞合成分泌ＮＧＦ的因子，而且该因子来源于巨噬细胞。Objective To study the influence of macrophages on NGF(nerve growth factor)secretion in adult rat Schwann cells Methods Several kinds of adult rat peritoneal macrophages conditioned media were treated on purified cultured Schwann cells for 24 hours,then measured the NGF contents in the cultured Schwann cells suspension media by a two cite enzymelinked immunoserbent assay(ELISA) Results Both degenerated myelin and lipopolysaccharide(LPS) activated M Φ CM enhanced NGF secreting in rat Schwann cells obviously Conclusions M Φ CM contain some factors which can promote NGF secretion in rat Schwann cells in vitro,it suggested that macrophages regulating NGF secretion by secreted certain cytokines

关 键 词：巨噬细胞 许旺细胞 神经生长因子 条件培养基 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]