拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析  

Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant

作　　者：刘秋萍[1,2] 曹华[3,2] 姚茂金[4,2] 马英[4,2] 邓斌生[4,2] 权军利[2] 单卫星[1,2] 

机构地区：[1]西北农林科技大学植物保护学院,杨凌712100 [2]西北农林科技大学陕西省农业分子生物学重点实验室,杨凌712100 [3]西北农林科技大学生命科学学院,杨凌712100 [4]西北农林科技大学农学院,杨凌712100

出　　处：《植物学报》2010年第5期548-555,共8页Chinese Bulletin of Botany

基　　金：国家大学生创新性实验计划(No.071071202);教育部新世纪优秀人才支持计划(No.NCET-05-0856);现代农业产业技术体系(No.nycytx-15)

摘　　要：非寄主抗病性是一种普遍的自然现象,该文通过建立拟南芥-大豆疫霉菌(Arabidopsis thaliana-Phytophthora sojae)非寄主互作系统,筛选对大豆疫霉菌感病的拟南芥突变体,为研究植物对卵菌的非寄主抗病性遗传机制奠定基础。以大豆疫霉菌游动孢子接种拟南芥T-DNA插入突变体离体叶片,从代表12000个独立转化株系的40000株T3代T-DNA插入拟南芥突变体中获得一系列对大豆疫霉菌感病的突变体。其中突变体581-51感病性状表现稳定,离体叶片接菌后3天内出现明显的水渍状病斑,4-5天后产生大量卵孢子和/或孢子囊。细胞学观察发现有典型的吸器形成。Southern杂交和遗传分析结果表明,581-51突变体含有4个T-DNA插入事件,其感病性状可能由隐性单基因控制。Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants.A collection of more than 40 000 T3 A.thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P.sojae zoospores,and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization.A number of P.sojae-susceptible A.thaliana mutants were successfully obtained,and one of them,mutant 581-51,was shown to be stably susceptible to P.sojae infection.Water-soaked lesions formed on the detached leaves within 3 days,as did oospores and sporangia 4-5 days after inoculation with P.sojae zoospores.Cytological characterization revealed the formation of haustoria-like structures.Southern analysis showed the presence of four T-DNA insertion events in the mutant.Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P.sojae in the mutant 581-51 was likely controlled by a single recessive gene.

关 键 词：拟南芥 非寄主抗病性 卵菌 大豆疫霉菌 T-DNA插入突变体 

分 类 号：S432.2[农业科学—植物病理学]