清热解毒方剂对脓毒症大鼠肠系膜淋巴结免疫功能的影响  

Effects of Qingrejiedu recipe on immune function of mesenteric lymph nodes in septic rats

作　　者：喻文立[1] 崔乃强[2] 傅强[2] 刘洪斌[3] 李东华[3] 

机构地区：[1]天津市第一中心医院,天津300192 [2]南开医院,天津300100 [3]天津市中西医结合急腹症研究所,天津300100

出　　处：《中国新药与临床杂志》2010年第9期696-699,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的观察清热解毒方剂对脓毒症大鼠肠系膜淋巴结(MLN)免疫功能的影响及作用机制。方法采用盲肠结扎穿孔方法制作大鼠脓毒症模型,健康雄性Wistar大鼠120只,随机分为假手术组、模型组和清热解毒方剂组,每组40只,分别于造模后0、6、12、24、72 h留取MLN组织,运用流式细胞仪测定MLN中T辅助细胞1(Th1)与T辅助细胞2(Th2)比值和调节性T细胞(Treg)占CD4+T细胞比例。结果与假手术组比较,模型组MLN Th1与Th2比值在造模后6 h升高,造模后12、24、72 h降低(P<0.05或P<0.01),而Treg比例在造模后6 h明显下降,随后明显升高(P<0.05或P<0.01),清热解毒方剂组Th1与Th2比值以及Treg比例升高和降低的幅度较模型组明显减低。在模型组和清热解毒方剂组,Th1与Th2比值与Treg比例呈线性负相关(r=-0.895和r=-0.926)。结论清热解毒方剂可能通过调节Treg比例来减少Th1向Th2的漂移,维持MLN局部细胞免疫功能,一定程度预防了脓毒症的发生。AIM To investigate the effects and relative mechanisms of Qingrejiedu（QRJD） recipe on immune function of mesenteric lymph nodes（MLN） in septic rats.METHODS Intra-abdominal infection was induced by cecum ligation and puncture（CLP）.One hundred and twenty male Wistar rats were randomly divided into sham group,model group and QRJD group,n = 40 respectively.MLN samples of each eight rats were collected at 0,6,12,24 and 72 h after CLP.The ratio of T helper cells 1 and T helper cells 2（Th1/Th2） and the percentage of regulatory T cells（Treg） in CD4＋ T cells in MLN were determined by flow cytometry.RESULTS The level of Th1/Th2 increased at 6 h after CLP,but decreased at 12,24,72 h in the model group（P 0.05 or P 0.01）,while the percentage of Treg in CD4＋ T cells was significantly decreased at 6 h and increased later in the model group as compared with the sham group.The QRJD group have similar trends of Th1/Th2 and the percentage of Treg in the model group,but with smaller changes.There were negative correlations between Th1/Th2 and the percentage of Treg both in the model group and in the QRJD group（r =-0.895 and r =-0.926,respectively）.CONCLUSION QRJD recipe can obviously increase percentage of Treg and decreased Th1/Th2,maintain MLN immune function and prevent the sepsis.

关 键 词：脓毒症 肠系膜 淋巴结 T淋巴细胞 辅助诱导 清热解毒方剂 

分 类 号：R285.5[医药卫生—中药学] R657.2[医药卫生—中医学]