FSHR胞外区抗原的二级结构预测和B细胞表位预测  

Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR

作　　者：申子刚[1] 阎萍[2] 何畏[2] 陈正琼[2] 何海洋[1] 张记[1] 杨霞[1] 吴玉章[1] 梁志清[2] 李晋涛[1] 

机构地区：[1]第三军医大学免疫学教研室 [2]第三军医大学西南医院妇产科,重庆400038

出　　处：《重庆医科大学学报》2010年第9期1317-1320,共4页Journal of Chongqing Medical University

基　　金：国家科技支撑计划资助项(编号:2006BAI03B12);国家自然基金资助项目(编号:30400402;30571708)

摘　　要：目的:分析卵泡刺激素受体(Follicle stimulating hormone receptor,FSHR)胞外区蛋白的二级结构,在线预测FSHR胞外区潜在的B细胞表位。方法:利用DNAStarProtein软件对FSHR胞外区蛋白的二级结构和表面特性进行分析,联合在线B细胞表位预测,预测FSHR胞外区蛋白可能的抗原表位。合成预测的B细胞表位肽,对其抗原性进行鉴定。结果:FSHR胞外区蛋白可能的抗原表位区域为17～34、42～44、116～122、142～147、179～193、239～241、266～270、279～282、288～307。选择4个区域合成肽段表位区域能与免疫抗血清发生抗原特异性反应。结论:应用多参数成功预测了FSHR胞外区蛋白抗原的B细胞表位。Objective：To predict the secondary structure and the B cell epitope for the extracellular domain of the follicle stimulating hormone receptor（FSHR）.Methods：The secondary structure and surface properties of the extracellular domain of FSHR,such as physical and chemical properties,hydrophilicity,accessibility,antigenicity and plasticity,were analyzed by a variety of methods.The peptide of the epitopes was synthesized and used for coating streptavidin plate.The immunogenicity of the peptides was determined.Results：Many distinct antigenic epitopes in the extracellular domain of FSHR were identified by computation and online prediction,the possible location of which is in the regions of 17~34、42~44、116~122、142~147、179~193、239~241、266~270、279~282、288~307.Synthesized peptides have high specificity to the polyclonal antiboby.Conclusion：The B cell epitope for the extracellular domain of FSHR is successfully predicted.

关 键 词：FSHR胞外区 B细胞表位 二级结构 抗原性 

分 类 号：R587.1[医药卫生—内分泌]