Bcl-2蛋白在外伤性癫痫病灶中的表达  

An immunohistochemical study on Bcl-2 in the cerebral focus of posttraumatic epilepsy(PTE)

作　　者：蔡卫林[1,2] 唐运林[3] 何鹏翔[1] 黄永华[1] 方超[1] 姜富学 竞花兰[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]中国人民武装警察部队浙江省消防总队医院,浙江杭州310011 [3]中国人民解放军第458医院,广东广州510000 [4]广州市刑事科学与技术研究所,广东广州510000

出　　处：《中国法医学杂志》2010年第5期312-314,318,共4页Chinese Journal of Forensic Medicine

摘　　要：目的观察Bcl-2蛋白在外伤性癫痫病灶中的表达变化,探讨其作用机制。方法收集外伤性癫痫、非外性癫痫患者及交通事故死亡者脑皮质各15例,采用免疫组织化学和荧光免疫组织化学技术,观察各实验组Bcl-2蛋白表达水平,测定阳性表达的灰度值,数据采用SPSS 12.0软件进行统计分析。结果外伤性及非外伤性癫痫组Bcl-2蛋白表达水平均高于交通事故死亡组(P<0.05);而非外伤性癫痫组表达明显高于外伤性癫痫组,差异亦具有显著统计学意义(P<0.05)。结论 Bcl-2蛋白能抑制神经元凋亡,对外伤性癫痫的发病过程具有抑制作用。Objective To observe change of Bcl-2 expression in the cerebral focus of posttraumatic epilepsy（PTE） and investigate its pathogenesis.Methods 15 cerebral tissue samples from the PTE patients,15 samples from non-PTE patients and 15 samples from bodies died of traffic accident were collected,respectively.Bcl-2 protein in were observed by immunohistochemical and immunofluorescent techniques.Gray scale of positive immunostaining were measured,and the data were statistically analyzed with SPSS for Windows 12.0.Results Bcl-2 immunoreactivity was stronger in cerebral foci in both PTE and non-PTE patients than in cortical tissues from bodies died of traffic accident.The intensity of Bcl-2 immunoreactivity was significantly stronger in non-PTE patients than in PTE patients（P〈0.05）.Conclusion The Bcl-2 expression may be up-regulated in the PTE cerebral focus,which may suppress the neuroapoptosis and alleviate the onset of PTE.

关 键 词：法医病理学 外伤性癫痫 Bcl-2 免疫组织化学染色 荧光免疫组织化学染色 

分 类 号：D919.4[医药卫生—法医学]