雷公藤内酯醇对阿尔茨海默病模型大鼠海马补体3表达的影响  

Effects of triptolide on complement 3 expression of hippocampus in Alzheimer＇s disease model rats

作　　者：胡小令[1] 吕诚[1] 杨宝林[1] 万斌[1] 聂菁[1] 温蔚[2] 石嘉庆[2] 

机构地区：[1]南昌大学医学院解剖学教研室,南昌330006 [2]南昌大学医学院医学实验教学部,南昌330006

出　　处：《解剖学杂志》2010年第5期660-663,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30660073）;江西省教育厅科技项目计划（赣教技字[2007]87号）

摘　　要：目的：观察β-淀粉样蛋白（Aβ）对大鼠海马补体3（C3）表达的变化及雷公藤内酯醇对其的影响.方法：SD雄性大鼠随机等分成对照组、模型组、用药组.在大鼠双侧海马定向注射凝聚态Aβ1-40作为模型组,注射等量生理盐水设为对照组,大鼠在海马注射凝聚态Aβ1-40后腹腔注射雷公藤内酯醇则为用药组.应用免疫组织化学、RT-PCR方法检测各组大鼠海马C3的表达.结果：免疫组织化学显色显示,模型组C3阳性细胞数与对照组比较明显增多、模型组C3阳性细胞平均光密度与对照组比较明显增高.用药组C3阳性细胞平均光密度较模型组明显降低.RT-PCR结果显示,模型组C3 mRNA的表达水平比对照组明显增高;而用药组C3 mRNA的表达水平低于模型组.结论：雷公藤内酯醇对Aβ所致的大鼠海马C3的活化有抑制作用.Objective： To explore the effects of triptolide （TP） and β-amyloid protein （AI3） on complement 3 （123） expression of hippoeampus in model rats with Alzheimer＇s disease （AD）. Methods： Thirty male SD rats were randomly divided into three equal groups： a control group, AD model group and TP-treated group. The AD model group was prepared with bilateral microinjection of aggregated Aβ1-40 into rat hippocampus while the control group was only bilaterally microinjected with normal saline. The TP-treated group was first bilaterally mieroinjeeted with aggregated Aβ1-40 into the hippocampus and then administered intraperitoneally with TP. The variations of C3 expression of hippocampus in these groups were observed by immuno-histoehemical staining and RT-PCR. Results： The cell number of the C3 positive staining in the AD model group （95.42±23. 45） was considerably higher than that in the control group （30. 09±6. 52） and the average optical density of the C3 positive staining in the AD model group （0. 335 1±0. 038 8） was greatly higher than that in the control group （0. 166 4±0. 016 1）. It was also found that the average optical density of the C3 positive staining in the TP-treated group （0. 272 1±0. 032 7） was significantly lower than that in the AD model group （0. 335 1±0. 038 8）. The RT-PCR gave comparable results that the expression level of C3 mRNA in the AD model group （0. 858±0. 023） was significantly higher than that in the control group （0. 671±0. 034） while the expression level of C3 mRNA in the TP-treated group （0. 772±0. 029） was noticeably lower than that in the model group （0. 858±0. 023）. Conclusion： TP can inhibit the activation of hippocampal C3 in the AD model rats.

关 键 词：雷公藤内酯醇 Β-淀粉样蛋白 阿尔茨海默病 补体3 海马 

分 类 号：R285.5[医药卫生—中药学]