肺移植术前后血清可溶性细胞间黏附分子-1检测的临床意义  

The clinical significance of serum level of soluble intercellular adhesion molecule-1 detected before and after lung transplantation

作　　者：肖华龙[1] 谢国强[1] 陈国千[1] 张晓峰[1] 强新晨[1] 虞竞峰[1] 朱岚[2] 祝炳方[1] 黄飚[2] 

机构地区：[1]江苏省无锡市人民医院检验科,214023 [2]卫生部核医学重点实验室、江苏省原子医学研究所

出　　处：《中华核医学杂志》2010年第5期343-345,共3页Chinese Journal of Nuclear Medicine

基　　金：基金项目：卫生部核医学重点实验室开放课题（WK005-021）

摘　　要：目的 探讨肺移植术前后血清可溶性细胞间黏附分子-1（sICAM-1）检测的临床意义及其对肺移植后发生急性排斥反应诊断的可能性.方法 采用生物素-链亲和素系统时间分辨荧光免疫分析（BSA-TRFIA）法检测26例肺移植受者手术前后血清sICAM-1的变化,将30名健康体格检查者（对照组）的sICAM-1分别与肺移植稳定组（16例）、急性排斥反应组（4例）和感染组（6例）进行比较,对各肺移植组之间sICAM-1进行比较.采用非参数秩和检验（Kruskal-Wallis H）和方差分析进行统计学比较.结果 肺移植术前各组sICAM-1[（357.07±220.07）、（396.18±136.25）、（468.95±85.48）μg/L]与对照组[（348.63±69.12）μg/L]差异无统计学意义（H=6.0436,P＞0.05） 术后各肺移植组与对照组sICAM-1差异有统计学意义[术后1周各肺移植组sICAM-1分别为（274.23±157.53）、（455.53±126.51）和（146.43±327.11）μg/L,H=21.8994,P＜0.01].发生急性排斥反应时血清sICAM-1升高,并发感染时sICAM-1显著降低,各肺移植组之间术后sICAM-1比较差异均无统计学意义（F=1.7820,P＞0.05）.结论 检测血清sICAM-1有助于肺移植急性排斥反应的诊断.Objective To evaluate serum soluble intercellular adhesion molecule-1 （sICAM-1） before and after lung transplantation for diagnosing acute rejection. Methods Biotin-streptavidin time resolved fluoroimmunoassay （BSA-TRFIA） was used to detect the concentration of serum sICAM-1 before and after lung transplantation in 26 patients. All patients were divided into stable lung transplantation group （n =16）, acute rejection group （n =4） and infected group （n =6）. The serum level of sICAM-1 in those groups was compared with that of the control group （ n = 30 ） by the non-parametric rank sum test （ KruskalWallis H test）. Results No significant difference was found for serum sICAM-1 among the three groups and the control group before operation： （357.07 ± 220.01 ）, （ 396. 18 ± 136.25 ）, （468.95 ± 85.48 ） μg/L vs（348.63 ±69. 12） μg/L, H=6. 0436, P 〉0.05. However, when rejection and infection happened after operation, the serum sICAM-1 increased in the acute rejection group （455.53 ± 126.51 μg/L） and decreased in the infection group （146.43 ± 327.11 μg/L）, and the level in the stable transplantation group was （274.23 ± 157.53 ） μg/L （H = 21. 8994, P 〈 0.01 ）. Conclusion Serum sICAM-1 level might be a potential marker to differentiate acute rejection from infection after lung transplantation.

关 键 词：肺移值 细胞粘着分子 荧光免疫测定 

分 类 号：R686[医药卫生—骨科学]