应用侧芽平切刻伤方法建立牡丹植株再生体系  

Establishment of Plantlet Regeneration System of Tree Peony Through Lateral Buds Cutting and Carving

作　　者：刘会超[1] 贾文庆[1] 

机构地区：[1]河南科技学院园林学院,河南新乡453003

出　　处：《园艺学报》2010年第9期1471-1476,共6页Acta Horticulturae Sinica

基　　金：河南省创新人才工程项目(2005-126-49);河南科技学院博士基金项目(050106);河南科技学院重点项目基金项目(050121)

摘　　要：以牡丹品种‘乌龙捧胜'5～6年生苗中下部饱满的侧芽为外植体,探讨了平切刻伤方法对侧芽诱导不定芽的影响,并研究了侧芽消毒方法、不同植物生长调节剂对不定芽继代增殖与生根的影响。结果表明:先用75%酒精消毒30s,再用0.1%升汞消毒7min,褐化率与污染率较低;采用平切刻伤的侧芽不定芽诱导率显著提高,诱导率为对照的2.6～4.8倍,最高达96%;不定芽增殖最佳的培养基为WPM+6-BA3.0mg·L-1+IAA0.2mg·L-1,增殖率可达到787.05%;生根最佳培养基为1/2改良MS+IBA4.0mg·L-1+NAA1.0mg·L-1,生根率达81.33%,移栽成活率为90%。Tree peony‘Wulong Pengsheng＇s lateral buds were used as explants.It was the first time to study the effect of cutting lateral buds on the adventitious buds differentiation.The influence of plant growth regulation on the adventitious buds induction,the multiplication and rooting was also studied.The results were as followed：（1）Disinfecting with 75% alcohol for 30 s,and then soaking with 0.1% corrosive sublimate for 7 min had good effect of disinfectation;（2）Compared with the control,the adventitious buds induction rate significantly increased through cutting,and the number of adventitious buds increased by 2.6–4.8 times,with the maximum reaching 96%;（3）The best medium for proliferation was WPM ＋ 6-BA mg · L^-1 ＋ IAA 0.2 mg · L^-1,with the proliferation of was 787.05%;（4）The rate of plantlets rooting was up to 81.33% on 1/2 WPM medium supplemented with NAA 1.0 mg · L^-1 and IBA 4.0 mg · L^-1.The plantlets were successfully transplanted to potting and the survival rate of transplanted plantlets was 90%.

关 键 词：牡丹 平切刻伤 不定芽 增殖 生根 

分 类 号：S685.11[农业科学—观赏园艺]