中国板栗种质资源的AFLP分析  

Analysis of Castanea mollissima Germplasm Resources by AFLP

作　　者：兰彦平[1] 周连第[1] 姚研武[1] 王尚德[1] 刘国彬[1] 

机构地区：[1]北京市农林科学院农业综合发展研究所,北京100097

出　　处：《园艺学报》2010年第9期1499-1506,共8页Acta Horticulturae Sinica

基　　金：北京市农林科学院常规育种财政专项;北京市科技新星计划项目(2005B32)

摘　　要：采用荧光标记AFLP技术,利用筛选出的9对EcoRⅠ/MseⅠ引物对90份中国板栗种质进行遗传变异及遗传关系分析。90份种质共产生806条带,695条多态性条带,多态比率86.23%;基于种质间遗传相似性系数的UPGMA聚类图将90份种质聚为两大类14个小组;9对引物在90个板栗种质中检测到数量不等的品种特异带型,各引物对对供试材料具有较高的鉴别效率,最高93%,最低80%。引物对两两组合可完全区分所有供试材料。The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations.Among the 806 bands detected, 695 bands were polymorphic,and the high percentage of polymorphic loci（86.23%）revealed a high genetic diversity among the 90 C.mollissima cultivars.The UPGMA phenogram based on genetic identity clustered the 90 C.mollissima cultivars into 2 groups and 14 rows.The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars,the highest ratio of identification is 93% and the lowest is 80%.

关 键 词：板栗 亲缘关系 AFLP 种质鉴定 

分 类 号：S664.2[农业科学—果树学]