辣椒花器官发育MADS-box基因的克隆与表达分析  

Cloning and Expression Analysis of a Flowering-related MADS-box Gene in Pepper

作　　者：郭爽[1,2] 马宁[1] 杨文才[1] 沈火林[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]广州市农业科学研究院,广州510308

出　　处：《园艺学报》2010年第10期1591-1597,共7页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2006BAD01A7;2008BADB1B04;2009BADB8B01);北京市教委共建项目;中央高校基本科研业务费专项资金项目(2009-2-06);果类蔬菜产业技术体系北京市创新团队项目

摘　　要：采用RACE技术从辣椒(Capsicum annuumL.)花药中获得了一个控制辣椒花器官发育的MADS-box基因,命名为PPI(GenBank登录号:GU812276)。该基因全长952bp,编码215个氨基酸,具有典型的MADS结构域和K结构域,与番茄花器官发育MADS-box基因TPI高度同源,同源性达88%。系统进化树分析表明,PPI基因属于花器官发育B类基因。RT-PCR表达分析表明,该基因在辣椒花瓣中的表达高于在花药、萼片以及子房等花器官中的表达,在营养器官叶片中没有表达。A flowering-related MADS-box gene named PPI was cloned from pepper anthers（Gen-Bank accession No.GU812276）using rapid amplification of cDNA ends.The full length cDNA of PPI was 952 bp with an open reading frame（ORF）encoding 215 amino acids.The deduced amino acids sequence contained a typical MADS domain and a K domain.Homology analysis showed that PPI had high homology to other MADS-box proteins from different species,especially 88% similarity to tomato TPI.Phylogenetic analysis indicated that PPI belonged to the MADS-box B subfamily.RT-PCR analysis showed that the expression of PPI gene was much stronger in petals than in anthers,sepals or ovaries,and no transcription was detected in leaves.

关 键 词：辣椒 花器官发育 MADS-BOX基因 PPI基因 RACE RT-PCR 

分 类 号：S641.3[农业科学—蔬菜学]