番茄SlIAA14基因启动子克隆及分析  

Cloning and Analysis of the Promoter of SlIAA14 in Tomato

作　　者：李小靖[1] 陈银华[1] 张俊红[2] 叶志彪[2] 

机构地区：[1]海南大学农学院,海口570228 [2]华中农业大学园艺林学学院,武汉430070

出　　处：《园艺学报》2010年第10期1605-1612,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金-青年科学基金项目(30800755)

摘　　要：为了深入研究SlIAA14基因的表达模式及其调控番茄根系发育的分子机理,利用染色体步移技术从番茄品种‘Ailsa Craig’中克隆到该基因上游2197bp的启动子片段。生物信息学分析表明,该启动子片段中含有多个对脱落酸、赤霉素、细胞分裂素等激素响应的顺式作用元件,以及对干旱和伤害等逆境胁迫响应的顺式作用元件。利用农杆菌介导的遗传转化方法获得SlIAA14::GUS+YFP融合基因的转基因番茄植株,转基因植株根系YFP和GUS检测结果显示,该启动子片段在番茄根尖和侧根原基有较高的表达活性,表明SlIAA14基因可能调控番茄根系的伸长和侧根发育的起始。Promoter analysis of SlIAA14 was carried out,to further study the expression profile of SlIAA14 and its regulatory mechanism in tomato root development.A 2 197 bp promoter fragment was cloned from tomato cultivar Ailsa Craig,using PCR-based genomic DNA walking.Several putative cis-elements responding to phytohormones（ABA,GA and CTK）and stress（dehydration and wounding） were predicted using online promoter analysis software.This promoter fragment was employed to drive YFP-GUS reporter genes,and was introduced into tomato using Agrobacterium-mediated transformation.YFP and GUS activity was detected in tomato root tip and lateral root primordium,suggesting SlIAA14 gene might participate in the root elongation and lateral root initiation of tomato.

关 键 词：番茄 SlIAA14 启动子 根系 发育 

分 类 号：S641.2[农业科学—蔬菜学]