胡萝卜小孢子胚状体和愈伤组织的诱导  

Induction of Microspores-derived Embryos and Calli from Anther Culture in Carrot

作　　者：庄飞云[1] 裴红霞[1] 欧承刚[1] 胡鸿[1] 赵志伟[1] 李金荣[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2010年第10期1613-1620,共8页Acta Horticulturae Sinica

基　　金：农业部‘948’项目(2006-G13);中央级公益性科研院所基本科研业务费专项(2060302-2-09);公益性行业科研(农业)专项(200903016);农业部园艺作物遗传改良重点开放实验室项目

摘　　要：选用胡萝卜7个常规品种、10个自交系、16个F1代杂交种和6个F2代材料研究基因型、植物生长调节剂、AgNO3以及低温预处理(4℃)对花药培养的影响。在参试的39份材料中有6份材料产生小孢子胚状体,其中50071号材料出胚率最高,达到3.89%,有30份材料产生小孢子愈伤组织,其中600Q6号材料出愈率达到36.70%。在B5培养基上添加2,4-D和NAA组合中,选用的4份参试材料均产生小孢子愈伤组织,其中50071和70Q67号材料产生胚状体,而添加6-BA和NAA组合中均未产生胚状体和愈伤组织。培养基中添加AgNO3对部分材料的出胚率有一定促进作用,50071和70Q67号材料在8mg·L-1AgNO3下出胚率分别达到10.00%和2.00%。低温预处理对部分材料的胚状体及小孢子愈伤组织形成有一定作用,50069号材料经4℃1d预处理出胚率达1%,预处理2d和3d出愈率均达到11.25%,70Q67号材料在3d预处理后出胚率达到4.00%。经细胞学鉴定,在121株由胚状体产生的再生植株中93.39%为二倍体,说明胡萝卜花药培养过程中易发生自然加倍。The influence of genotype,hormone,AgNO3 and low temperature （4 ℃） on anther culture of carrot （Daucus carota L.） was investigated with 7 open pollinated cultivars,10 inbred lines,16 F1 and 6 F2.Microspore embryogenesis occurred on six accessions and the highest frequency was 50071 with 3.89%.Thirty accessions formed the calli derived from microspores and 600Q6 had the highest frequency with 36.70%.On the B5 media with 2,4-D and NAA,all of four tested accessions formed the calli,and both of 50071 and 70Q67 formed the microspores-derived embryos,but none on the media with 6-BA and NAA.50071 and 70Q67 were induced about 10.00% and 2.00% of embryos on the media with 8 mg · L-1 AgNO3,respectively.Low temperature was effective on some accessions to form embryos and calli.50069 had the embryo ratio of 1% under the treatment of 1 d,and the callus ratio of 11.25% under the treatment of 2 d and 3 d,respectively.70Q67 had the embryo ratio of 4.00% with 3 d treatment.About 93.39% plants were diploid among 121 plantlets regenerated from embryos with cytogenetic analysis,which was regarded as the result of spontaneous diploidization during anther culture.

关 键 词：胡萝卜 花药培养 胚状体 小孢子愈伤组织 自然加倍 

分 类 号：S631.2[农业科学—蔬菜学]