单表达和共表达NDVF和HN基因重组鸡痘病毒活疫苗的免疫效力比较试验  

Protective efficacy of recombinant fowlpox viruses expressing Newcastle disease virus F gene or HN gene or co-expressing F and HN genes

作　　者：甘军纪[1] 田志鹏[1] 陶鸽[1] 陈素娟[1] 彭大新[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国预防兽医学报》2010年第10期790-794,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划(2006AA10A205);江苏省高技术研究计划(BG2007336);江苏省自然科学基金(SBK200930422)

摘　　要：为评价母源抗体对抗新城疫病毒(NDV)重组鸡痘病毒(rFPV)活疫苗免疫效力的影响,本研究用单表达、共表达NDV基因VII型病毒ZJ1株F、HN基因的rFPV活疫苗rFPV-12LSF、rFPV-12LSHN、rFPV-12LSFHN和油乳剂灭活疫苗分别免疫14日龄商品蛋鸡,rFPV疫苗的免疫剂量均为2×104pfu,免疫后21d分别用106ELD50NDV不同强毒株攻毒。其中F48E8株攻毒的免疫保护率分别为30.3%、73.2%、41.1%;而ZJ1株攻毒的免疫保护率分别为89.3%和35.8%、67.9%、78.6%、100%。用表达鸡IL-2基因的rFPV-12LSIL-2与rFPV-12LSHN联合免疫没有提高rFPV-12LSHN疫苗的免疫保护力。试验结果表明,单表达NDV HN基因的rFPV-12LSHN可作为NDV活疫苗候选株,为NDV重组鸡痘病毒活疫苗的进一步应用性开发奠定了基础。The protective efficacy of recombinant fowlpox viurses（rFPV） against Newcastle disease（NDV） were investigated.Two-week-old commercial layer chickens were inoculated with rFPVs expressing either F gene（rFPV-12LSF） or HN gene（rFPV-12LSHN） of NDV strain ZJ1,or rFPV co-expressing F and HN genes（rFPV-12LSFHN） or inactivated vaccine,and challenged with virulent NDV strain F48E8 or ZJ1 at 21 days post inoculation.The protective rate of rFPV-12LSF,rFPV-12LSHN,rFPV-12LSFHN and inactivated vaccines were 30.3%,73.2%,41.1% and 89.3%,respectively,against F48E8,and 35.8%,67.9%,78.6% and 100%,respectively against ZJ1.A recombinant fowlpox virus expressing chicken IL-2（rFPV-12LSIL-2） did not improve the protective efficacy of rFPV-12LSHN when combined with rFPV-12LSHN.The results indicated that the rFPV-12LSHN could be an effective candidate vaccine against NDV.

关 键 词：重组鸡痘病毒 F基因 HN基因 疫苗 新城疫 

分 类 号：S852.65[农业科学—基础兽医学]