禽流感病毒H5N1变异株灭活疫苗(Re-4株)对鸡、鸭和鹅的免疫效果研究  

The protective efficacy of the inactivated H5N1 subtype avian influenza virus vaccine Re-4 in chickens,ducks and geese

作　　者：曾显营[1] 钟功勋[1] 李雁冰[1] 施建忠[1] 姜永萍[1] 王彦妮[1] 万晓鹏[1] 毛胜刚[2] 田国彬[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江哈尔滨150001 [2]中国农业科学院哈尔滨兽医研究所哈尔滨维科生物技术开发公司,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第10期800-803,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划(2006BAD06A05;2006BAD06A16);哈尔滨市科技攻关项目(2007AA3BN030);现代农业产业技术体系建设(nycytx-42-G3-02)

摘　　要：为系统评估禽流感病毒(AIV)H5N1变异株灭活疫苗(Re-4株)对家禽的免疫效果,本研究将Re-4株油乳剂灭活疫苗免疫SPF鸡和商品蛋鸡、商品鸭及商品鹅。免疫后每周采集血清测定HI抗体,绘制抗体消长曲线,免疫SPF鸡在免疫后2周、3周和50周时以105EID50剂量的强毒株(CK/SX/2/06)进行攻毒。研究结果显示,该疫苗对蛋鸡、鸭、鹅均具有良好的免疫效果,而且SPF免疫鸡血清HI抗体在4log2以上时能够完全抵抗CK/SX/2/06强毒的攻击。因此,根据实验结果推荐该油乳剂灭活疫苗的对上述禽类的免疫程序:商品蛋鸡10日龄颈部皮下注射0.3mL,60日龄和110日龄(开产前)时依次胸肌注射0.5mL和1.0mL进行免疫;商品鸭、鹅在2周龄均以0.5mL首免,5周龄和4月龄左右时以1mL的剂量肌肉注射方式进行加强免疫。The protective efficacy of the inactivated avian influenza virus（AIV） H5N1 subtype vaccine（Re-4） derived from AIV H5N1 A/Chicken/Shanxi/2/2006（CK/SX/2/06） were evaluated in chickens,ducks and geese.Chickens,ducks and goose were immunized with Re-4 vaccine and the hemagglutinin-inhibition（HI） antibody was detected on a weekly basis.The SPF chickens were intranasally challenged with 105 EID50 of CK/SX/2/06 at 2,3 and 50 weeks post vaccination（p.v）.The results showed that Re-4 vaccine induced strong immune response in chickens,ducks and geese.SPF chickens with a HI antibody titre above 4 log2 were completely protected from challenge.Based on these results,we propose the following vaccination program：layers could be immunized with the initial dose of 0.3 mL at the age of 10-day-old and the second dose of 0.5 mL at the age of 60-day-old,andthe third dose of 1.0 mL at the age of 110-day-old;ducks and geese could be immunized with the initial dose of 0.5 mL at the age of 2-week-old and the second dose of 1.0 mL at the age of 5-week-old,and the third dose of 1.0 mL at the age of 4-month-old.

关 键 词：禽流感 H5N1 变异株 灭活疫苗 免疫效果 

分 类 号：S852.65[农业科学—基础兽医学]