犬弓首蛔虫ITS1基因的克隆及序列分析  

Cloning and sequencing of ITS1 rDNA from Toxocara canis

作　　者：周荣琼[1,2] 夏庆友[2] 黄汉成[1] 邓梦竹[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460 [2]西南大学蚕学与系统生物研究所,重庆400716

出　　处：《中国预防兽医学报》2010年第10期818-820,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：重庆市自然科学基金项目(CSTC;2009BB113);西南大学荣昌校区重点学科项目

摘　　要：通过对国内5个犬弓首蛔虫株(T.canis)即GD株、CQ株、YN株、HN株和GZ株的rDNA的内转录间隔区Ⅰ(ITS1)序列进行PCR扩增、克隆、测序和序列分析,旨在确定ITS1是否可作为T.canis分子分类的遗传标记。结果显示:GD株、CQ株、YN株、HN株和GZ株的ITS1序列基本一致,仅GD株有2个碱基的差异,HN株和GY株分别有1个碱基的差异;与GenBank中登录的T.canis(序列号为AB110024、AB110026)的ITS1序列同源性高达98.9%～99.4%。结果表明ITS1可作为分子标记用于T.canis与其它蛔虫的种间鉴定,但不适合用于T.canis种内遗传变异的研究,为进一步的分子遗传学和分子诊断学研究奠定基础。The aims of this study were to analyze the genetic variations among Toxocara canis isolates from different origins in China,and to establish foundation for further studies on molecular genetics and diagnostics of T.canis.The first internal transcribed spacer（ITS1） of rDNA from GD,CQ,YN,HN and GZ strains of T.canis were amplified by PCR,cloned and sequenced.The results showed that the ITS1 sequences of GD,CQ,YN,HN and GZ strains were near identical with 98.9% to 99.4% identities compared with the published T.cani sequences.The ITS1 sequences of the GD,HN and GZ strains differed by two bases,one base and one base,respectively compared with that of the other 4 isolates.The results indicated that ITS1 sequences provide useful for the identification and differentiation of T.canis from other related roundworms,but the sequences are not suitable for studying the genetic variation within T.canis.

关 键 词：犬弓首蛔虫 PCR ITS1 

分 类 号：S852.65[农业科学—基础兽医学]