普通小麦-柔软滨麦草易位系M8657-1抗条锈病基因遗传分析与SSR标记  

Genetic Analysis and SSR Location of Stripe Rust Resistance of Wheat Translocation Line M8657-1 Derived from Triticum aestivum-Leymus mollis(Trin.) Hara

作　　者：杨敏娜[1,2] 彭岳林[1] 姚强[2] 贺苗苗[2] 井金学[2] 

机构地区：[1]西藏农牧学院,林芝860000 [2]西北农林科技大学植物保护学院/陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《农业生物技术学报》2010年第5期861-866,共6页Journal of Agricultural Biotechnology

基　　金：教育部高等学校学科创新引智计划项目(No.B07049);教育部长江学者和创新团队发展计划(No.200558);国家“十一五”攻关计划(No.2006BAD08A05);国家自然科学基金(No.30960214);陕西省科技攻关项目(No.2005k02-G1-1)共同资助

摘　　要：为明确小麦(Triticum aestivum)-柔软滨麦草(Leymus mollis(Trin.)Hara)易位系M8657-1的抗条锈性,用中国小麦条锈菌(Puccinia striiform f.sp.stritici)流行小种条中30号、条中31号、水源11-4和水源11-11生理小种,对M8657-1和铭贤169的杂交后代进行苗期抗条锈性遗传分析。结果表明,易位系M8657-1对条中30号和水源11-11的抗条锈性均1对隐性核基因控制;对条中31号的抗条锈性由2对显性核基因(互补作用)控制;对水源11-4的抗条锈性由1对显性核基因控制。将控制水源11-4抗病性的基因暂时命名为YrElm1-4,以接种水源11-4的F2正交群体为研究对象,应用BSA法进行了SSR分析。从320对SSR引物组合中筛选到3个与主效抗病基因YrElm1-4连锁的多态性微卫星标记,它们分别是Xgwm636、Xwmc522和Xwmc453,根据3个微卫星标记位点的染色体位置,推出YrElm1-4位于小麦2AS染色体上,这3个标记可用于分子标记辅助育种。To identify and tag new resistance genes to stripe rust in a translocation line M8657-1（commom wheat（Triticum aestivum） translocated with Leymus mollis（Trin.） Hara）,the yellow rust races CYR30,CYR31,SU11-4 and SU11-11 were seleceted as inoculum.Genetic analysis of resistance to stripe rust was made in the progeny population of M8657-1/Mingxian169 at seedling stage.The results showed that one recessive gene conferred resistance to stripe rust race CYR30 and SU11-11,respectively;Two dominant karyoplasm genes conferred resistance to CYR31;And the resistance to SU11-4 was controlled by one dominant gene which was temporarily designated as YrElm1-4.The F2 population inoculated with SU11-4 were used for SSR analysis.SSR markers,as well as 320 pair primers,combined with bulked segregation analysis（BSA） revealed that three SSR markers,Xgwm636,Xwmc522 and Xwmc453,located on the short arm of chromosome 2A were linked to YrElm1-4.And YrElm1-4 was deduced to locate on the chromosome 2AS based on the three markers location.So the three SSR markers can be used in molecular-asisted breeding.

关 键 词：小麦-柔软滨麦草易位系 小麦条锈病 抗病基因 遗传分析 SSR标记 

分 类 号：S512.1[农业科学—作物学]