济宁青山羊高繁殖力候选基因类固醇21-羟化酶基因(CYP21)的研究  

Steroid 21-hydroxylase Gene(CYP21) as a Candidate Gene for Prolificacy of Jining Grey Goat

作　　者：闫艳[1] 王凭青[1] 储明星[2] 古丽格娜[3] 狄冉[2] 方丽[2] 马月辉[2] 李奎[2] 

机构地区：[1]重庆大学生物工程学院,重庆400030 [2]中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与利用重点开放实验室,北京100193 [3]新疆畜牧科学院畜牧研究所,乌鲁木齐830000

出　　处：《农业生物技术学报》2010年第5期917-924,共8页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)(No.2006AA10Z139);国家现代农业产业技术体系建设专项资金(农科教发[2008]10号);国家重点基础研究发展计划(973)(No.2006CB102105);国家自然科学基金项目(No.30871773);中国农业科学院北京畜牧兽医研究所基本科研业务费专项(No.2010jc-9);中国农业科学院首批优秀科技创新团队专项共同资助

摘　　要：采用PCR-SSCP和聚丙烯酰胺凝胶电泳(PAGE)技术检测类固醇21-羟化酶基因(steroid 21-hydroxylase,CYP21)10个外显子在山羊(Capra hircus)低繁殖力品种(安哥拉山羊和内蒙古绒山羊)、中等繁殖力品种(波尔山羊)和高繁殖力品种(济宁青山羊)中的碱基变异,同时研究该基因对济宁青山羊高繁殖力的影响。结果表明,10对引物中仅引物P10扩增片段存在多态性。对于P10扩增产物,在内蒙古绒山羊、波尔山羊和济宁青山羊中检测到AA、AB和BB基因型,在安哥拉山羊中检测到AA和AB基因型;测序分析发现,BB与AA基因型相比发生了A2789C、C2791A、G2818A、G2851C、G2852T和G2854A的突变,在第2821与2822位发生了两个碱基CG的缺失突变,导致426～448位共有19个氨基酸发生改变;济宁青山羊BB和AB基因型产羔数最小二乘均值分别比AA基因型的多0.81只(P<0.05)和0.47只(P<0.05),BB和AB基因型之间差异不显著(P>0.05)。研究结果初步表明CYP21基因的B等位基因是提高山羊产羔数的一个潜在有效的DNA标记。The steroid 21-hydroxylase gene（CYP21） was studied as a candidate gene for the high prolificacy in Jining Grey goats.According to the sequence of bovine CYP21 gene,ten pairs of primers were designed to detect base variation of exons 1～10 of CYP21 gene in goat（Capra hircus） low prolificacy breeds（Angora and Inner Mongolia Cashmere goats）,medium prolificacy breed（Boergoat） and high prolificacy breed（Jining Grey goat） by PCR-SSCP and PAGE.Only the products amplified by primer P10 displayed polymorphisms.For primer P10,three genotypes（AA,AB and BB） were detected in Inner Mongolia Cashmere,Boer and Jining Grey goats,and two genotypes（AA and AB） were detected in Angora goats.Sequencing revealed six nucleotide mutations（A2789C,C2791A,G2818A,G2851C,G2852T and G2854A） and one deletion mutation（c.2821_2822delCG）at exon 10 of CYP21 gene in the BB genotype compared with the AA genotype.As a result,nineteen amino acids were changed from the 426th to the 448th.The Jining Grey goats with genotype BB and AB had 0.81（P0.05） and 0.47（P0.05） kids more than those with genotype AA,and the difference of the littersize between genotypes BB and AB was non-significant（P0.05）.These results preliminarily indicate that allele B of CYP21 gene is a potential DNA markerforimproving littersize in goats.

关 键 词：山羊 高繁殖力 类固醇21-羟化酶基因(CYP21) PCR-SSCP 

分 类 号：S827.8[农业科学—畜牧学]