利用SSR及EST标记对鲤鱼饲料转化率的QTL分析  

QTL Analysis Related to Feed Conversion Efficiency in Common Carp(Cyprinus carpio) Using SSR and EST Markers

作　　者：张丽博[1,2] 张晓峰[1] 曹顶臣[1] 杨晶[1,3] 储致远[1,3] 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070 [2]上海海洋大学水产与生命技术学院,上海201306 [3]大连水产学院生命科学与技术学院,大连116023

出　　处：《农业生物技术学报》2010年第5期963-967,共5页Journal of Agricultural Biotechnology

基　　金：国家重大基础研究发展规划(973)项目(No.2004CB117405);农业部"引进国际先进农业科学技术"计划(No.2007-G55(A))共同资助

摘　　要：利用137个SSR(simple sequence repeat)及EST(expressed sequence tag)标记,对柏氏鲤(Cyprinus pellegrini)/荷包红鲤(Cyprinus carpio wuyuanensis)抗寒品系的BC1F1群体93个个体进行了基因型检测。利用Windows Map Manager2.0的标记回归法进行了饲料转化率数量性状单标记回归分析。检测结果得到了8个微卫星标记(HLJ133、HLJ360、HLJ380、HLJ562、HLJ740、HLJ852、HLJ930和HLJ1420)和2个EST标记(HLJE318和HLJE417)与鲤鱼饲料转化率相关,对性状的表型贡献率为4%～11%。对同一标记的不同基因型间饲料转化率性状进行了显著性比较,通过ANOVA检验找到了相关基因型,标记HLJ360、HLJ380、HLJ562座位上,分别对应基因型212/212、236/224、220/220与饲料转化率性状正相关。本研究检测出的与鲤鱼饲料转化率性状相关的标记及基因型,为鲤鱼的QTL定位及分子标记辅助育种(MAS)提供了有效的依据。One hundred and thirty seven SSR（simple sequence repeat） and EST（expressed sequence tag） markers were used to analyze the genotype of 93 individuals of BC1F1 generation which cross from Barbless carp（Cyprinus pellegrini） and Hebao-cold tolerance red carp（Cyprinus carpio wuyuanensis）.Using Windows Map Manager 2.0 software analyzed the quantitative trait related to feed conversion efficiency by single marker regression analysis.Results showed that eight SSR markers（HLJ133,HLJ360,HLJ380,HLJ562,HLJ740,HLJ852,HLJ930 and HLJ1420） and two EST markers（HLJE318 and HLJE417） were discovered related to common crap＇s feed conversion ratio.The phenotype variation was explained by these loci,ranged from 4% to 11%.The genotypes of these correlative loci were determined by ANOVA analysis.In loci HLJ360、HLJ380 and HLJ562 respectively,genotypes 212/212,236/224 and 220/220 are positive correlation to feed conversion efficiency trait.These functional markers and genotypes which related to common crap＇s feed conversion may be used forthe QTL mapping and markerassisted selection in common carp.

关 键 词：鲤鱼 饲料转化率 SSR EST QTL分析 

分 类 号：S831.5[农业科学—畜牧学]