水貂细胞色素b基因(Cytb)克隆、序列分析及与鼬属动物的进化关系  

Cloning and Sequence Analysis of Mitochondrial Cytochrome b Gene(Cytb) in Mink(Mustela vison) and Its Evolutionary Relationships with the Mustela

作　　者：涂剑锋[1] 王雷[1] 邢秀梅[1] 李一清[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《农业生物技术学报》2010年第5期975-980,共6页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划项目(No.2006BAD06B06);国家自然科技资源平台项目(No.2004DKA30460)共同资助

摘　　要：参照水貂(Mustela vison)近缘物种线粒体基因组序列设计引物,通过克隆、测序、再拼接的方法获得6个水貂品种和左家型水貂线粒体Cytb基因全序列。用DNAstar v6.13和DnaSP4.0分析显示,水貂Cytb基因全长为1140bp,平均碱基含量为29.8%A、13.0%G、30.3%T及26.9%C,检测到12个多态位点,全为转换。用ClustalX1.8进行多序列比较分析表明,水貂个体间Cytb基因相似性非常高。结合GenBank检索到的14种鼬属动物同源序列,用MEGA4.0基于邻接法分别构建鼬属动物核苷酸序列和氨基酸序列系统进化树,两种进化树得出相似的拓扑结构。结果表明:我国水貂与美洲水貂亲缘较近,与欧洲水貂(M.lutreola)亲缘较远,推算美洲水貂与欧洲水貂分歧时间在中新世。A pair of primers were designed by referring to species close to mink（Mustela.vison）,the complete mitochondrial cytochrome b gene（Cytb） sequences of six mink breeds and Zuojia mink were determined using cloning,sequenceing and assembling.Sequence analysis used DNAstar v6.13 and DnaSP4.0 revealed that Cytb Gene of minks were 1 140 bp in length,the composition of nucleotides A,G,T and C was 29.8%,13.0%,30.3% and 26.9%,respectively.Twelve variable sites were detected,and they all were transition.Multiple sequences alignments by ClustalX1.8 showed that they were high similarity in Cytb gene among minks.Combined with homologous sequences of 14 species in the Mustela retrieved from GenBank,the phylogenetic trees based on nucleotide and amino acid sequences were constructed by Neighbor-joining method,respectively.Two trees had same topologies.The results indicated that minks in China had closer evolutionary relationships with American minks than with European mink（M.lutreola）.The divergence time between American mink and European mink might have started at Miocene.

关 键 词：水貂 CYTB基因 克隆 序列分析 进化关系 

分 类 号：S828.2[农业科学—畜牧学]