聚乙二醇修饰的壳聚糖包裹DNA的转染效果研究  

Transfection Efficiency of the DNA Nanoparticles Wrapped with PEG Modified Chitosan

作　　者：蔡文萍[1] 李轶杰[1] 

机构地区：[1]新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物技术》2010年第5期29-32,共4页Biotechnology

基　　金：国家自然科学基金项目(30860265);新疆生物资源基因工程重点实验室基金项目(No.XJDX0201-2005-02)资助

摘　　要：目的:为探讨聚乙二醇(PEG,MW4000)修饰的壳聚糖用于包裹质粒DNA(Chi-DNA)是否能有效提高口服发送的外源基因在消化道中的表达量。方法:用PEG修饰的壳聚糖包裹不同剂量(10μg,50μg,100μg)的质粒(pCMVβ),形成壳聚糖纳米复合物,通过口服发送后经X-gal染色法检测pCMVβ在小鼠消化道内的表达效果。结果:胃酸消化实验及DNaseⅠ消化实验均表明经PEG修饰的壳聚糖纳米粒能有效地保护DNA,使其免受胃酸及DnaseⅠ的降解。同时在小鼠消化道内的表达量较修饰前明显增加,随着所发送DNA剂量的增加,表达量也相应增加,且包裹10μg质粒的DNA纳米复合物的表达量相当于100μg未修饰复合物的表达水平。结论:经PEG修饰的Chi-DNA纳米复合物有较高的基因转染效果,有望作为基因治疗中高效非病毒口服发送系统的材料。Objective：To investigate the efficiency of of the DNA nanoparticles wrapped with PEG modified chitosan by oral delivery.Method：Various dosage （10μg,50μg,100μg,respectively） of plasmid pCMVβ were enwrapped in the modified chitosan,and administrated by oral route to Kunming white mice.The alimentary canal was stained with X-Gal according to standard protocols to investigate the efficiency of DNA transfection.Result：The results showed that transfection efficiency of chitosan-DNA nanoparticles modified with PEG was apparently enhanced than that without modification.With the amount of delivered DNA increased,the expression of the DNA in alimentary canal increased accordingly.And the mice oral administrated with the 10μg of modified chitosan-DNA nanoparticles exhibited the same transfection efficiency as that with 100μg of unmodified chitosan-DNA.Conclusion：The PEG modified chitosan wraped DNA had high transfection efficiency.It might be a potential way for oral delivery of non-viral vector system in gene therapy.

关 键 词：聚乙二醇(PEG) 壳聚糖 Chi-DNA纳米复合物 口服 转染效果 

分 类 号：R944[医药卫生—药剂学]