骆驼蓬籽蛋白提取物体外抑癌活性的研究  

Study on the Antitumor Activity of Protein Extracts of Peganalaum harmala Seeds in vitro

作　　者：罗晶晶[1] 马晓瑾[1] 殷晓丽[1] 陈勇[1] 孙素荣[1] 

机构地区：[1]新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物技术》2010年第5期32-34,共3页Biotechnology

基　　金：新疆自治区科技支疆项目(2009911219);新疆高校科研计划重点项目(XJEDU2008I01);新疆生物资源基因工程重点实验室开放基金项目(XJDX0201-2009-03)资助

摘　　要：目的:探讨骆驼蓬籽蛋白提取物的体外抑癌活性。方法:采用MTT法检测硫酸铵沉淀的骆驼蓬籽蛋白提取物体外抗肿瘤活性,应用流式细胞术和Annexin V/PI双荧光染色法检测其对细胞凋亡的影响。结果:80%硫酸铵饱和度下的蛋白提取物对宫颈癌HeLa、食道癌Eca109和肝癌BEL-7404细胞的IC50分别为72.11±3.88μg/mL、257.58±1.16μg/mL和174.07±1.32μg/mL。对HeLa细胞的抑制率与空白对照组相比有显著性差异(P<0.01),呈现较好的量效关系,且有促进细胞凋亡的作用。结论:骆驼蓬籽蛋白中对HeLa细胞增殖具有较好的抑制活性,可能是通过诱导细胞凋亡来发挥其抗肿瘤作用。Objective：To evaluate antitumor efficacy of the protein extracts of Peganalaum harmala seeds in vitro.Method：The crude proteins of Peganum harmala L.seeds were extracted by saturated ammonium sulfate.Cell growth inhibitory effects of the crude protein extracts on tumor cells in vitro were examined with MTT assay.Cell apoptosis was detected by flow cytometry （FCM） and annexin V/PI fluorescence staining.Result：The 80% saturation of ammonium sulfate from Peganalaum harmala seeds protein had the cell inhibition activity against HeLa,Eca109,and BEL-7404 cells,IC50 were 72.11±3.88μg/mL,257.58±1.16μg/mL,and 174.07±1.32μg/mL,respectively.It showed significantly cell inhibition activity against HeLa cell （P0.01）,and induced apoptosis in a dose-dependent manner.Conclusion：The Peganalaum harmala seeds protein could significantly inhibit HeLa growth and induce its apoptosis.

关 键 词：骆驼蓬 HELA细胞 抑癌活性 细胞凋亡 

分 类 号：R284.2[医药卫生—中药学] Q964.91[医药卫生—中医学]