一种通用的基因组DNA提取方法  

A Universal Extraction Method for Genomic DNA

作　　者：张俊河[1] 王芳[1] 房娟[2] 曹冰睿[2] 李云[2] 郭彦梅[2] 向梅[2] 王天云[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,河南新乡453003 [2]新乡医学院基础医学院,本科2007级河南新乡453003

出　　处：《生物技术》2010年第5期34-36,共3页Biotechnology

基　　金：河南省科技攻关项目(0624410041);河南省自然科学基金项目(2008A310008)资助

摘　　要：目的:探讨一种通用的基因组DNA提取方法。方法:采用改良的膜法分别从动植物组织、外周血、细菌、细胞等标本提取基因组DNA,DNA样品经紫外吸收、琼脂糖凝胶电泳、PCR扩增和酶切进行检测。结果:该方法提取的基因组DNA纯度较高,电泳条带清晰,DNA质量能满足下游分子生物学研究的需要。结论:该方法简便快速、适用范围广,是提取基因组DNA的一种有效方法。Objective：To investigate a universal method for extracting genomic DNA.Method：An improved silica-membrane method was used to extract the genomic DNA from animal and plant tissues,peripheral blood,bacteria and cell.DNA was evaluated through UV spectrophotometer analysis,agarose gel electrophoresis,PCR and restriction enzyme digestion respectively.Result：Higher purity genomic DNA was obtained and it could meet the need of molecular biology manipulation.Conclusion：The method is simple,rapid and can be widely used in different samplesgenomic DNA extraction.

关 键 词：基因组DNA PCR 提取 

分 类 号：Q78[生物学—分子生物学]