响应曲面法优化小牛胸腺肽提取工艺  

Optimized Technology for Extracting Thymosin by Response Surface Methodology

作　　者：林莹[1] 钟恒禄[2] 沈前程[2] 黄海愉[2] 张为宇[2] 

机构地区：[1]广西大学轻工与食品学院,广西南宁530004 [2]广西大学动物科学技术学院,广西南宁530005

出　　处：《生物技术》2010年第5期62-66,共5页Biotechnology

摘　　要：目的:为了模拟并且优化小牛胸腺肽提取工艺,获得良好的活性肽提取效果。方法:在单因素试验的基础上,采用响应曲面法对影响胸腺肽提取率的四个因素即料液比、匀浆时间、匀浆液pH值和冻融次数进行优化。利用DesignExpert7.0软件对胸腺肽提取率的二次多项数学模型分析。结果:在液料比1.94:1、匀浆时间3.38min、pH值3.23、冻融次数7次时,胸腺肽提取率达最大值,最佳提取率预测值为8.874mg/g,实测值为8.349mg/g。结论:采用响应曲面法能有效地优化小牛胸腺肽提取工艺,获得理想的提取效果。Objective：To model and optimize the extraction method to obtain favourable active peptide.Method：On the basis of single-factor experiments,a four-factor,three-level Box-Behnken design combining with response surface methodology （RSM） was employed to optimize four crucial extraction conditions for maximizing yield of thymosin.A regression model was established for yield of Thymosin as a function of the four extraction conditions,the analysis for this model by Design Expert 7.0 software.Result：The optimal ratio of water to material,the time ,the pH of homogenate and the time of freeze thaw were1：1.94g/ml,3.38min,3.23 and 7 times,respectively.Under these conditions,the predicted value of yield of thymosin was 8.874mg/ml,and the actual value was 8.349mg/ml.Conclusion：Response surface methodology （RSM） was employed to optimize the extraction technology for thymosin and obtained effective extraction.

关 键 词：胸腺肽 提取 优化 响应曲面法 

分 类 号：Q503[生物学—生物化学]